Alpha-fetoprotein Matched ELISA Antibody Pair Set,Human

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Alpha-fetoprotein Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
Alpha-fetoprotein Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human Alpha-fetoprotein
线性范围
31.25-2000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 1 mg/mL of mouse anti-Human AFP / alpha-fetoprotein monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 12177-MM33)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human AFP / alpha-fetoprotein monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in Dilution Buffer before use. (Catalog: # 12177-MM26)
3. Standard Each vial contains 115 ng of recombinant Human AFP / alpha-fetoprotein. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 2000 pg/mL is recommended.
产品概述
This Alpha-fetoprotein Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Alpha-fetoprotein . It contains Human Alpha-fetoprotein capture antibody, Human Alpha-fetoprotein detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Alpha-fetoprotein protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Alpha-fetoprotein Matched ELISA Antibody Pair Set,Human 图片

Alpha-fetoprotein Matched ELISA Antibody Pair Set,Human: 别称

AFPD Matched ELISA Antibody Pair Set, Human; Alpha-fetoprotein Matched ELISA Antibody Pair Set, Human; FETA Matched ELISA Antibody Pair Set, Human; HPAFP Matched ELISA Antibody Pair Set, Human

Alpha-fetoprotein 背景信息

Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
全称
alpha-fetoprotein
参考文献
  • Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
  • Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
  • Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
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