carbonic anhydrase XII Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
carbonic anhydrase XII Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
carbonic anhydrase XII Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human carbonic anhydrase XII
线性范围
7.81-500 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 90 μg/mL of mouse anti-CA12 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating.
2. Detection Antibody 0.25 mg/mL mouse anti-CA12 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 37 ng of recombinant CA12. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.5 ng/mL is recommended.
2. Detection Antibody 0.25 mg/mL mouse anti-CA12 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 37 ng of recombinant CA12. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.5 ng/mL is recommended.
产品概述
This carbonic anhydrase XII Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human carbonic anhydrase XII . It contains Human carbonic anhydrase XII capture antibody, Human carbonic anhydrase XII detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human carbonic anhydrase XII protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
carbonic anhydrase XII Matched ELISA Antibody Pair Set,Human 图片
carbonic anhydrase XII Matched ELISA Antibody Pair Set,Human: 别称
CA12 Matched ELISA Antibody Pair Set, Human; CAXII Matched ELISA Antibody Pair Set, Human; FLJ20151 Matched ELISA Antibody Pair Set, Human; HsT18816 Matched ELISA Antibody Pair Set, Human
carbonic anhydrase XII 背景信息
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
全称
carbonic anhydrase XII
参考文献
- Sahin, U. et al., 1996, Proc. Natl. Acad. Sci. U.S.A. 92 (25): 11810–11813.
- Ivanov, S.V. et al., 1998, Proc. Natl. Acad. Sci. USA 95:12596 - 12601.
- Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 16903.
- Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
- Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
- Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
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