CD14 Matched ELISA Antibody Pair Set,Mouse

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CD14 Matched ELISA Antibody Pair Set,Mouse 产品信息

产品名称
CD14 Matched ELISA Antibody Pair Set,Mouse
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Mouse CD14
线性范围
78.13-5000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 0.5 mg/mL of mouse anti-mouse CD14 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 50023-R217)
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse CD14 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50023-R002)
3. Standard Each vial contains 90 ng of recombinant mouse CD14. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.
产品概述
This CD14 Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD14 . It contains Mouse CD14 capture antibody, Mouse CD14 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse CD14 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

CD14 Matched ELISA Antibody Pair Set,Mouse 图片

CD14 Matched ELISA Antibody Pair Set,Mouse: 别称

CD14 Matched ELISA Antibody Pair Set, Mouse

CD14 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
全称
CD14 molecule
Related Pathways
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • SD Wright, et al. (1990) CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science. 249 (4975): 1431-3.
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