CD147 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
CD147 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
CD147 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human CD147
线性范围
6.25-400 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of mouse anti-CD147 monoclonal antibody, Dilute to a working concentration of 4.0 μg/mL in CBS before coating. (Catalog: # 10186-MM05)
2. Detection Antibody 0.5 mg/mL rabbit anti-CD147 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.125 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10186-R118)
3. Standard Each vial contains 25 ng of recombinant CD147. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
2. Detection Antibody 0.5 mg/mL rabbit anti-CD147 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.125 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10186-R118)
3. Standard Each vial contains 25 ng of recombinant CD147. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
产品概述
This CD147 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human CD147 . It contains Human CD147 capture antibody, Human CD147 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human CD147 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CD147 Matched ELISA Antibody Pair Set,Human 图片
CD147 Matched ELISA Antibody Pair Set,Human: 别称
5F7 Matched ELISA Antibody Pair Set, Human; Basigin Matched ELISA Antibody Pair Set, Human; CD147 Matched ELISA Antibody Pair Set, Human; EMMPRIN Matched ELISA Antibody Pair Set, Human; M6 Matched ELISA Antibody Pair Set, Human; OK Matched ELISA Antibody Pair Set, Human; TCSF Matched ELISA Antibody Pair Set, Human
CD147 背景信息
CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.
全称
basigin (Ok blood group)
参考文献
- Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.
- Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.
- Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.
- Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.
- Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.
- Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.
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