CD31 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
CD31 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
CD31 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human CD31
线性范围
18.75-12000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of mouse anti-Human CD31/PECAM1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10148-MM06)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human CD31/PECAM1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10148-MM12)
3. Standard Each vial contains 615 ng of recombinant Human CD31/PECAM1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 12000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human CD31/PECAM1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10148-MM12)
3. Standard Each vial contains 615 ng of recombinant Human CD31/PECAM1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 12000 pg/mL is recommended.
产品概述
This CD31 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human CD31 . It contains Human CD31 capture antibody, Human CD31 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human CD31 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CD31 Matched ELISA Antibody Pair Set,Human 图片
CD31 Matched ELISA Antibody Pair Set,Human: 别称
CD31 Matched ELISA Antibody Pair Set, Human; CD31/EndoCAM Matched ELISA Antibody Pair Set, Human; endoCAM Matched ELISA Antibody Pair Set, Human; GPIIA' Matched ELISA Antibody Pair Set, Human; PECA1 Matched ELISA Antibody Pair Set, Human; PECAM-1 Matched ELISA Antibody Pair Set, Human
CD31 背景信息
The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.
全称
platelet/endothelial cell adhesion molecule 1
参考文献
- Deaglio S, et al. (2000) CD38/CD31, a receptor/ligand system ruling adhesion and signaling in human leukocytes. Chem Immunol. 75: 99-120.
- Kohler S, et al. (2009) Life after the thymus: CD31+ and CD31- human naive CD4+ T-cell subsets. Blood. 113(4): 769-74.
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