CD50 Matched ELISA Antibody Pair Set,Human

For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
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CD50 Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
CD50 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human CD50
线性范围
62.5-4000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 0.5 mg/mL of mouse anti-ICAM3 monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10333-MM02)
2. Detection Antibody 0.5 mg/mL mouse anti-ICAM3 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 40 ng of recombinant ICAM3. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
产品概述
This CD50 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human CD50 . It contains Human CD50 capture antibody, Human CD50 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human CD50 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

CD50 Matched ELISA Antibody Pair Set,Human 图片

CD50 Matched ELISA Antibody Pair Set,Human: 别称

CD50 Matched ELISA Antibody Pair Set, Human; CDW50 Matched ELISA Antibody Pair Set, Human; ICAM-3 Matched ELISA Antibody Pair Set, Human; ICAM-R Matched ELISA Antibody Pair Set, Human

CD50 背景信息

The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting of three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes and is thus an important molecule for the first immune response. ICAM-3 comprises five immunoglobulin-like domains and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule but also as a potent signaling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that the CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. Also, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase in cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.
全称
intercellular adhesion molecule 3
参考文献
  • Berney SM, et al. (1999) ICAM-3 (CD50) cross-linking augments signaling in CD3-activated peripheral human T lymphocytes. J Leukoc Biol. 65(6): 867-74.
  • van Buul JD, et al. (2004) ICAM-3 activation modulates cell-cell contacts of human bone marrow endothelial cells. J Vasc Res. 41(1): 28-37.
  • Sugino H. (2005) ICAM-3, a ligand for DC-SIGN, was duplicated from ICAM-1 in mammalian evolution, but was lost in the rodent genome. FEBS Lett. 579(13): 2901-6.
  • Park JK, et al. (2010) ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB. Int J Oncol. 36(1): 181-92.
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