CD59 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
CD59 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
CD59 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human CD59
线性范围
234.38-15000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of rabbit anti-Human CD59 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 12474-R015)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human CD59 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 12474-R031)
3. Standard Each vial contains 240 ng of recombinant Human CD59. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human CD59 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 12474-R031)
3. Standard Each vial contains 240 ng of recombinant Human CD59. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 15000 pg/mL is recommended.
产品概述
This CD59 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human CD59 . It contains Human CD59 capture antibody, Human CD59 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human CD59 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CD59 Matched ELISA Antibody Pair Set,Human 图片
CD59 Matched ELISA Antibody Pair Set,Human: 别称
16.3A5 Matched ELISA Antibody Pair Set, Human; 1F5 Matched ELISA Antibody Pair Set, Human; EJ16 Matched ELISA Antibody Pair Set, Human; EJ30 Matched ELISA Antibody Pair Set, Human; EL32 Matched ELISA Antibody Pair Set, Human; FLJ38134 Matched ELISA Antibody Pair Set, Human; FLJ92039 Matched ELISA Antibody Pair Set, Human; G344 Matched ELISA Antibody Pair Set, Human; HRF-20 Matched ELISA Antibody Pair Set, Human; HRF20 Matched ELISA Antibody Pair Set, Human; MAC-IP Matched ELISA Antibody Pair Set, Human; MACIF Matched ELISA Antibody Pair Set, Human; MEM43 Matched ELISA Antibody Pair Set, Human; MGC2354 Matched ELISA Antibody Pair Set, Human; MIC11 Matched ELISA Antibody Pair Set, Human; MIN1 Matched ELISA Antibody Pair Set, Human; MIN2 Matched ELISA Antibody Pair Set, Human; MIN3 Matched ELISA Antibody Pair Set, Human; MIRL Matched ELISA Antibody Pair Set, Human; MSK21 Matched ELISA Antibody Pair Set, Human; p18-20 Matched ELISA Antibody Pair Set, Human
CD59 背景信息
CD59 glycoprotein, also known as 2 kDa homologous restriction factor, HRF2, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
全称
CD59 molecule
Related Pathways
-
Complement Activation Pathways
参考文献
- Fletcher CM. et al., 1994, Structure. 2: 185-99.
- Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44.
- Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81.
- Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9.
- Picariello G. et al., 2008, Proteomics 8: 3833-47.
- Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.
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