CD8 alpha Matched ELISA Antibody Pair Set,Mouse
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
CD8 alpha Matched ELISA Antibody Pair Set,Mouse 产品信息
产品名称
CD8 alpha Matched ELISA Antibody Pair Set,Mouse
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Mouse CD8 alpha
线性范围
46.88-3000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of rabbit anti-mouse CD8a monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 50389-R016)
2. Detection Antibody 0.4 mg/mL rabbit anti-mouse CD8a polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 40 ng of recombinant mouse CD8a. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.
2. Detection Antibody 0.4 mg/mL rabbit anti-mouse CD8a polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 40 ng of recombinant mouse CD8a. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended.
产品概述
This CD8 alpha Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD8 alpha . It contains Mouse CD8 alpha capture antibody, Mouse CD8 alpha detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Mouse CD8 alpha protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CD8 alpha Matched ELISA Antibody Pair Set,Mouse 图片
CD8 alpha Matched ELISA Antibody Pair Set,Mouse: 别称
BB154331 Matched ELISA Antibody Pair Set, Mouse; Ly-2 Matched ELISA Antibody Pair Set, Mouse; Ly-35 Matched ELISA Antibody Pair Set, Mouse; Ly-B Matched ELISA Antibody Pair Set, Mouse; Lyt-2 Matched ELISA Antibody Pair Set, Mouse
CD8 alpha 背景信息
T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
全称
CD8a molecule
参考文献
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References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.
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