CD82 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
CD82 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
CD82 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human CD82
线性范围
78.13-5000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of rabbit anti-CD82 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 12275-R071)
2. Detection Antibody 0.17 mg/mL rabbit anti-CD82 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 67 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 12275-R072)
3. Standard Each vial contains 47 ng of recombinant CD82. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.
2. Detection Antibody 0.17 mg/mL rabbit anti-CD82 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 67 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 12275-R072)
3. Standard Each vial contains 47 ng of recombinant CD82. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.
产品概述
This CD82 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human CD82 . It contains Human CD82 capture antibody, Human CD82 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human CD82 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CD82 Matched ELISA Antibody Pair Set,Human 图片
CD82 Matched ELISA Antibody Pair Set,Human: 别称
4F9 Matched ELISA Antibody Pair Set, Human; C33 Matched ELISA Antibody Pair Set, Human; GR15 Matched ELISA Antibody Pair Set, Human; IA4 Matched ELISA Antibody Pair Set, Human; KAI-1 Matched ELISA Antibody Pair Set, Human; KAI1 Matched ELISA Antibody Pair Set, Human; R2 Matched ELISA Antibody Pair Set, Human; SAR2 Matched ELISA Antibody Pair Set, Human; ST6 Matched ELISA Antibody Pair Set, Human; TSPAN27 Matched ELISA Antibody Pair Set, Human
CD82 背景信息
CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.
全称
CD82 molecule
参考文献
- Malik FA, et al. (2009) KAI-1/CD82, the molecule and clinical implication in cancer and cancer metastasis. Histol Histopathol. 24(4): 519-30.
- Liu WM, et al. (2006) KAI1/CD82, a tumor metastasis suppressor. Cancer Lett. 240(2): 183-94.
- Tonoli H, et al. (2006) CD82 metastasis suppressor gene: a potential target for new therapeutics? Trends Mol Med. 11(12): 563-70.
- Jackson P, et al. (2005) KAI1 tetraspanin and metastasis suppressor. Int J Biochem Cell Biol. 37(3): 530-4.
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