Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human Carboxypeptidase B1
线性范围
6.25-400 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.2 mg/mL of mouse anti-CPB1 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10501-MM06)
2. Detection Antibody 0.5 mg/mL of rabbit anti-CPB1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10501-R108)
3. Standard Each vial contains 30 ng of recombinant CPB1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
2. Detection Antibody 0.5 mg/mL of rabbit anti-CPB1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10501-R108)
3. Standard Each vial contains 30 ng of recombinant CPB1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
产品概述
This Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Carboxypeptidase B1 . It contains Human Carboxypeptidase B1 capture antibody, Human Carboxypeptidase B1 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human Carboxypeptidase B1 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human 图片
Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human: 别称
CPB Matched ELISA Antibody Pair Set, Human; PASP Matched ELISA Antibody Pair Set, Human; PCPB Matched ELISA Antibody Pair Set, Human
Carboxypeptidase B1 背景信息
Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
全称
carboxypeptidase B1 (tissue)
参考文献
- Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
- Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
- Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
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