Quantitative determination of Human Carboxypeptidase B1
1. Capture Antibody 0.2 mg/mL of mouse anti-CPB1 monoclonal antibody, Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10501-MM06) 2. Detection Antibody 0.5 mg/mL of rabbit anti-CPB1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10501-R108) 3. Standard Each vial contains 30 ng of recombinant CPB1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
This Carboxypeptidase B1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Carboxypeptidase B1 . It contains Human Carboxypeptidase B1 capture antibody, Human Carboxypeptidase B1 detector antibody
and a highly purified
recombinant Human Carboxypeptidase B1 protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
carboxypeptidase B1 (tissue)
Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.