DDR1 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
DDR1 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
DDR1 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human DDR1
线性范围
56.25-3600 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of rabbit anti-Human DDR1 / MCK10 / CD167 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10730-R205)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human DDR1 / MCK10 / CD167 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10730-R017)
3. Standard Each vial contains 155 ng of recombinant Human DDR1 / MCK10 / CD167. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3600 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human DDR1 / MCK10 / CD167 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10730-R017)
3. Standard Each vial contains 155 ng of recombinant Human DDR1 / MCK10 / CD167. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3600 pg/mL is recommended.
产品概述
This DDR1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human DDR1 . It contains Human DDR1 capture antibody, Human DDR1 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human DDR1 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
DDR1 Matched ELISA Antibody Pair Set,Human 图片
DDR1 Matched ELISA Antibody Pair Set,Human: 别称
CAK Matched ELISA Antibody Pair Set, Human; CD167 Matched ELISA Antibody Pair Set, Human; DDR Matched ELISA Antibody Pair Set, Human; EDDR1 Matched ELISA Antibody Pair Set, Human; HGK2 Matched ELISA Antibody Pair Set, Human; MCK10 Matched ELISA Antibody Pair Set, Human; NEP Matched ELISA Antibody Pair Set, Human; NTRK4 Matched ELISA Antibody Pair Set, Human; PTK3 Matched ELISA Antibody Pair Set, Human; PTK3A Matched ELISA Antibody Pair Set, Human; RTK6 Matched ELISA Antibody Pair Set, Human; TRKE Matched ELISA Antibody Pair Set, Human
DDR1 背景信息
Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
全称
discoidin domain receptor tyrosine kinase 1
Related Pathways
-
p53 Pathway
参考文献
- Hou G, et al. (2001) The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair. J Clin Invest. 107(6): 727-35.
- Ongusaha PP, et al. (2003) p53 induction and activation of DDR1 kinase counteract p53-mediated apoptosis and influence p53 regulation through a positive feedback loop. EMBO J. 22(6): 1289-301.
- Jönsson M, et al. (2001) Repression of Wnt-5a impairs DDR1 phosphorylation and modifies adhesion and migration of mammary cells. J Cell Sci. 114(11): 2043-53.
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