1. Capture Antibody 0.5 mg/mL of mouse anti-Human DKK1 / Dkk-1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 10170-MM08) 2. Detection Antibody 0.2 mg/mL of mouse anti-Human DKK1 / Dkk-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10170-MM03) 3. Standard Each vial contains 20 ng of recombinant Human DKK1 / Dkk-1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
This DKK1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human DKK1 . It contains Human DKK1 capture antibody, Human DKK1 detector antibody
and a highly purified
recombinant Human DKK1 protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
DKK1 Matched ELISA Antibody Pair Set,Human 图片
DKK1 Matched ELISA Antibody Pair Set,Human: 别称
DKK-1 Matched ELISA Antibody Pair Set, Human; SK Matched ELISA Antibody Pair Set, Human
Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.