DR4/TRAIL R1 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
DR4/TRAIL R1 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
DR4/TRAIL R1 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human DR4/TRAIL R1
线性范围
2.34-150 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of mouse anti-Human TRAIL R1 / CD261 / TNFRSF10A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10408-MM01)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human TRAIL R1 / CD261 / TNFRSF10A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10408-R001)
3. Standard Each vial contains 18 ng of recombinant Human TRAIL R1 / CD261 / TNFRSF10A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 150 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human TRAIL R1 / CD261 / TNFRSF10A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10408-R001)
3. Standard Each vial contains 18 ng of recombinant Human TRAIL R1 / CD261 / TNFRSF10A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 150 pg/mL is recommended.
产品概述
This DR4/TRAIL R1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human DR4/TRAIL R1 . It contains Human DR4/TRAIL R1 capture antibody, Human DR4/TRAIL R1 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human DR4/TRAIL R1 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
DR4/TRAIL R1 Matched ELISA Antibody Pair Set,Human 图片
DR4/TRAIL R1 Matched ELISA Antibody Pair Set,Human: 别称
APO2 Matched ELISA Antibody Pair Set, Human; CD261 Matched ELISA Antibody Pair Set, Human; DR4 Matched ELISA Antibody Pair Set, Human; MGC9365 Matched ELISA Antibody Pair Set, Human; TNFRSF10A Matched ELISA Antibody Pair Set, Human; TRAILR-1 Matched ELISA Antibody Pair Set, Human; TRAILR1 Matched ELISA Antibody Pair Set, Human
DR4/TRAIL R1 背景信息
Tumor necrosis factor receptor superfamily, member 10a (TRAIL R1), also known as TRAIL receptor 1 (TRAIL R1) or CD261 antigen, is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R1/CD261/TNFRSF10A serves as a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAIL R1 can promote the activation of NF-kappa-B. TRAIL R1/CD261/TNFRSF10A induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types.
全称
tumor necrosis factor receptor superfamily member 10a
研究领域
Related Pathways
-
Death Receptor Signaling
参考文献
- Kimberley FC, et al. (2005) Following a TRAIL: update on a ligand and its five receptors.". Cell Res. 14 (5): 359–72.
- Schneider P, et al. (1997) Characterization of two receptors for TRAIL.". FEBS Lett. 416 (3): 329–34.
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