EpCAM Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
EpCAM Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
EpCAM Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human EpCAM
线性范围
15.625-1000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of mouse anti-Human EpCAM/TROP-1/TACSTD1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10694-MM07)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human EpCAM/TROP-1/TACSTD1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10694-R028)
3. Standard Each vial contains 180 ng of recombinant Human EpCAM/TROP-1/TACSTD1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human EpCAM/TROP-1/TACSTD1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10694-R028)
3. Standard Each vial contains 180 ng of recombinant Human EpCAM/TROP-1/TACSTD1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
产品概述
This EpCAM Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human EpCAM . It contains Human EpCAM capture antibody, Human EpCAM detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human EpCAM protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
EpCAM Matched ELISA Antibody Pair Set,Human 图片
EpCAM Matched ELISA Antibody Pair Set,Human: 别称
DIAR5 Matched ELISA Antibody Pair Set, Human; EGP-2 Matched ELISA Antibody Pair Set, Human; EGP314 Matched ELISA Antibody Pair Set, Human; EGP40 Matched ELISA Antibody Pair Set, Human; ESA Matched ELISA Antibody Pair Set, Human; HNPCC8 Matched ELISA Antibody Pair Set, Human; KS1/4 Matched ELISA Antibody Pair Set, Human; KSA Matched ELISA Antibody Pair Set, Human; M4S1 Matched ELISA Antibody Pair Set, Human; MIC18 Matched ELISA Antibody Pair Set, Human; MK-1 Matched ELISA Antibody Pair Set, Human; TACSTD1 Matched ELISA Antibody Pair Set, Human; TROP-1 Matched ELISA Antibody Pair Set, Human; TROP1 Matched ELISA Antibody Pair Set, Human
EpCAM 背景信息
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
全称
epithelial cell adhesion molecule
参考文献
- Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
- Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
- Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
- Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
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