EpCAM Matched ELISA Antibody Pair Set,Mouse
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
EpCAM Matched ELISA Antibody Pair Set,Mouse 产品信息
产品名称
EpCAM Matched ELISA Antibody Pair Set,Mouse
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Mouse EpCAM
线性范围
78.13-5000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1.0 mg/mL of rabbit anti-mouse EPCAM monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 50591-R052)
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse EPCAM monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50591-R002)
3. Standard Each vial contains 400 ng of recombinant mouse EPCAM. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.
2. Detection Antibody 0.5 mg/mL rabbit anti-mouse EPCAM monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50591-R002)
3. Standard Each vial contains 400 ng of recombinant mouse EPCAM. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5 ng/mL is recommended.
产品概述
This EpCAM Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse EpCAM . It contains Mouse EpCAM capture antibody, Mouse EpCAM detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Mouse EpCAM protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
EpCAM Matched ELISA Antibody Pair Set,Mouse 图片
EpCAM Matched ELISA Antibody Pair Set,Mouse: 别称
CD326 Matched ELISA Antibody Pair Set, Mouse; EGP Matched ELISA Antibody Pair Set, Mouse; EGP-2 Matched ELISA Antibody Pair Set, Mouse; Egp314 Matched ELISA Antibody Pair Set, Mouse; Ep-CAM Matched ELISA Antibody Pair Set, Mouse; EpCAM1 Matched ELISA Antibody Pair Set, Mouse; GA733-2 Matched ELISA Antibody Pair Set, Mouse; gp40 Matched ELISA Antibody Pair Set, Mouse; Ly74 Matched ELISA Antibody Pair Set, Mouse; Tacsd1 Matched ELISA Antibody Pair Set, Mouse; Tacstd1 Matched ELISA Antibody Pair Set, Mouse; TROP1 Matched ELISA Antibody Pair Set, Mouse
EpCAM 背景信息
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
全称
epithelial cell adhesion molecule
参考文献
- Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
- Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
- Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
- Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
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