HE4 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
HE4 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
HE4 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human HE4
线性范围
15.625-1000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of mouse anti-Human HE4 / WFDC2 / WAP5 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 12609-MM12)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human HE4 / WFDC2 / WAP5 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.05 μg/mL in detection antibody dilution buffer before use. (Catalog: # 12609-R108)
3. Standard Each vial contains 50 ng of recombinant Human HE4 / WFDC2 / WAP5. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human HE4 / WFDC2 / WAP5 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.05 μg/mL in detection antibody dilution buffer before use. (Catalog: # 12609-R108)
3. Standard Each vial contains 50 ng of recombinant Human HE4 / WFDC2 / WAP5. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
产品概述
This HE4 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human HE4 . It contains Human HE4 capture antibody, Human HE4 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human HE4 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
HE4 Matched ELISA Antibody Pair Set,Human 图片
HE4 Matched ELISA Antibody Pair Set,Human: 别称
dJ461P17.6 Matched ELISA Antibody Pair Set, Human; EDDM4 Matched ELISA Antibody Pair Set, Human; HE4 Matched ELISA Antibody Pair Set, Human; WAP5 Matched ELISA Antibody Pair Set, Human
HE4 背景信息
WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein that contains two WAP domains. WFDC2 / HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2 / HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.
全称
WAP four-disulfide core domain 2
参考文献
- Bingle,L. et al., 2002, Oncogene. 21 (17):2768-73.
- Hellström,I. et al., 2003, Cancer Res. 63 (13):3695-700.
- Bingle,L. et al., 2006, Respir Res. 7 : 61.
- Galgano,M.T. et al., 2006, Mod Pathol.19 (6):847-53.
- Sharp,J.A. et al., 2007, Evol Dev. 9 (4): 378-92.
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