1. Capture Antibody 1 mg/mL of mouse anti-Human Hemopexin / HPX monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10870-MM08) 2. Detection Antibody 0.4 mg/mL of rabbit anti-Human Hemopexin / HPX polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard Each vial contains 48 ng of recombinant Human Hemopexin / HPX. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1200 pg/mL is recommended.
This Hemopexin Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Hemopexin . It contains Human Hemopexin capture antibody, Human Hemopexin detector antibody
and a highly purified
recombinant Human Hemopexin protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.