1. Capture Antibody 1.0 mg/mL of rabbit anti-HSP27 monoclonal antibody, Dilute to a working concentration of 1 μg/mL in CBS before coating. (Catalog: # 10351-R017) 2. Detection Antibody 0.5 mg/mL mouse anti-HSP27 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10351-MM01) 3. Standard Each vial contains 13 ng of recombinant HSP27. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.6 ng/mL is recommended.
This HSPB2 / HSP27 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human HSPB2 / HSP27 . It contains Human HSPB2 / HSP27 capture antibody, Human HSPB2 / HSP27 detector antibody
and a highly purified
recombinant Human HSPB2 / HSP27 protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.