ICOS ligand Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
ICOS ligand Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
ICOS ligand Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human ICOS ligand
线性范围
62.5-4000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of mouse anti-Human ICOS Ligand / B7-H2 / ICOSLG monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11559-MM05)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human ICOS Ligand / B7-H2 / ICOSLG monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11559-MM07)
3. Standard Each vial contains 82 ng of recombinant Human ICOS Ligand / B7-H2 / ICOSLG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human ICOS Ligand / B7-H2 / ICOSLG monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11559-MM07)
3. Standard Each vial contains 82 ng of recombinant Human ICOS Ligand / B7-H2 / ICOSLG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
产品概述
This ICOS ligand Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human ICOS ligand . It contains Human ICOS ligand capture antibody, Human ICOS ligand detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human ICOS ligand protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
ICOS ligand Matched ELISA Antibody Pair Set,Human 图片
ICOS ligand Matched ELISA Antibody Pair Set,Human: 别称
B7-h2 Matched ELISA Antibody Pair Set, Human; B7H2 Matched ELISA Antibody Pair Set, Human; B7RP-1 Matched ELISA Antibody Pair Set, Human; B7RP1 Matched ELISA Antibody Pair Set, Human; CD275 Matched ELISA Antibody Pair Set, Human; GL50 Matched ELISA Antibody Pair Set, Human; ICOS ligand Matched ELISA Antibody Pair Set, Human; ICOS-L Matched ELISA Antibody Pair Set, Human; ICOSL Matched ELISA Antibody Pair Set, Human; ICOSLG Matched ELISA Antibody Pair Set, Human; LICOS Matched ELISA Antibody Pair Set, Human
ICOS ligand 背景信息
Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation.
- Cancer Immunotherapy
- Co-stimulatory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: FCM Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
全称
inducible T-cell co-stimulator ligand
研究领域
参考文献
- Flesch IE. (2002) Inducible costimulator-ligand (ICOS-L). J Biol Regul Homeost Agents. 16(3): 217-9.
- Kajiwara K, et al. (2009) Expression and function of the inducible costimulator ligand B7-H2 in human airway smooth muscle cells. Allergol Int. 58(4): 573-83.
- Wong SC, et al. (2009) Functional hierarchy and relative contribution of the CD28/B7 and ICOS/B7-H2 costimulatory pathways to T cell-mediated delayed-type hypersensitivity. Cell Immunol. 256(1-2): 64-71.
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