IL-1 alpha/IL1A Matched ELISA Antibody Pair Set,Mouse
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
IL-1 alpha/IL1A Matched ELISA Antibody Pair Set,Mouse 产品信息
产品名称
IL-1 alpha/IL1A Matched ELISA Antibody Pair Set,Mouse
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Mouse IL-1 alpha/IL1A
线性范围
6.25-400 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of mouse anti-Mouse IL-1 alpha / IL-1F1 / IL1A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50114-R148)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse IL-1 alpha / IL-1F1 / IL1A polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 30 ng of recombinant Mouse IL-1 alpha / IL-1F1 / IL1A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse IL-1 alpha / IL-1F1 / IL1A polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 30 ng of recombinant Mouse IL-1 alpha / IL-1F1 / IL1A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
产品概述
This IL-1 alpha/IL1A Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse IL-1 alpha/IL1A . It contains Mouse IL-1 alpha/IL1A capture antibody, Mouse IL-1 alpha/IL1A detector antibody
(HRP)
and a highly purified
E. coli-expressed
recombinant Mouse IL-1 alpha/IL1A protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
IL-1 alpha/IL1A Matched ELISA Antibody Pair Set,Mouse 图片
IL-1 alpha/IL1A Matched ELISA Antibody Pair Set,Mouse: 别称
Il-1a Matched ELISA Antibody Pair Set, Mouse
IL-1 alpha/IL1A 背景信息
IL-1 alpha is a member of the interleukin 1 cytokine family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. Cytokines can be classified into two groups: pro- and anti-inflammatory. Pro-inflammatory cytokines, including IFNgamma, IL-1, IL-6, and TNF-alpha, are predominantly derived from the innate immune cells and Th1 cells. Anti-inflammatory cytokines, including IL-10, IL-4, IL-13, and IL-5, are synthesized from Th2 immune cells. IL-1 alpha is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. It is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. IL-1 alpha stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity.
全称
interleukin 1, alpha
研究领域
Related Pathways
-
IL1 signaling pathway
参考文献
- Nicklin MJ,et al. (1994) A physical map of the region encompassing the human interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist genes. Genomics. 19(2):382-4.
- March CJ, et al. (1985) Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs. Nature. 315(6021):641-7.
- Bankers-Fulbright JL, et al. (1996) Interleukin-1 signal transduction. Life Sci. 59(2):61-83.
- Dinarello CA, et al. (1997) Induction of interleukin-1 and interleukin-1 receptor antagonist. Semin Oncol. 24 (3 Suppl 9):S9-81-S9-93.
If you couldn't find the specific citation, you can:
