IL-18 Matched ELISA Antibody Pair Set,Mouse
IL-18 Matched ELISA Antibody Pair Set,Mouse 产品信息
产品名称
IL-18 Matched ELISA Antibody Pair Set,Mouse
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Mouse IL-18
线性范围
31.25-2000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse IL-18 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50073-R169)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse IL-18 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.3 μg/mL in Dilution Buffer before use. (Catalog: # 50073-R167)
3. Standard Each vial contains 64 ng of recombinant Mouse IL-18. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 2000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse IL-18 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.3 μg/mL in Dilution Buffer before use. (Catalog: # 50073-R167)
3. Standard Each vial contains 64 ng of recombinant Mouse IL-18. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 2000 pg/mL is recommended.
产品概述
This IL-18 Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse IL-18 . It contains Mouse IL-18 capture antibody, Mouse IL-18 detector antibody
(HRP)
and a highly purified
E. coli-expressed
recombinant Mouse IL-18 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
IL-18 Matched ELISA Antibody Pair Set,Mouse 图片
IL-18 Matched ELISA Antibody Pair Set,Mouse: 别称
Igif Matched ELISA Antibody Pair Set, Mouse; Il-18 Matched ELISA Antibody Pair Set, Mouse
IL-18 背景信息
Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.
全称
interleukin 18
研究领域
Related Pathways
-
IL1 signaling pathway
参考文献
- Dinarello CA. (1999) IL-18: A TH1-inducing, proinflammatory cytokine and new member of the IL-1 family. J Allergy Clin Immunol. 103: 11-24.
- Takeda K, et al.. (1998) Defective NK cell activity and Th1 response in IL-18-deficient mice. Immunity. 8(3): 383-90.
- Gracie JA, et al.. (1999) A proinflammatory role for IL-18 in rheumatoid arthritis. J Clin Invest. 104(10): 1393-401.
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An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS)AuthorLin, L;Xu, L;Lv, W;Han, L;Xiang, Y;Fu, L;Jin, M;Zhou, R;Chen, H;Zhang, A;Year2019JournalPLoS Pathog.ApplicationELISA
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