IL-32 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
IL-32 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
IL-32 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human IL-32
线性范围
23.44-1500 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1.0 mg/mL of rabbit anti-IL32A monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11064-R034)
2. Detection Antibody 0.2 mg/mL rabbit anti-IL32A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11064-R403)
3. Standard Each vial contains 33 ng of recombinant IL32A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5 ng/mL is recommended.
2. Detection Antibody 0.2 mg/mL rabbit anti-IL32A monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11064-R403)
3. Standard Each vial contains 33 ng of recombinant IL32A. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5 ng/mL is recommended.
产品概述
This IL-32 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human IL-32 . It contains Human IL-32 capture antibody, Human IL-32 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human IL-32 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
IL-32 Matched ELISA Antibody Pair Set,Human 图片
IL-32 Matched ELISA Antibody Pair Set,Human: 别称
IL-32alpha Matched ELISA Antibody Pair Set, Human; IL-32beta Matched ELISA Antibody Pair Set, Human; IL-32delta Matched ELISA Antibody Pair Set, Human; IL-32gamma Matched ELISA Antibody Pair Set, Human; IL32 Matched ELISA Antibody Pair Set, Human; NK4 Matched ELISA Antibody Pair Set, Human; TAIF Matched ELISA Antibody Pair Set, Human; TAIFa Matched ELISA Antibody Pair Set, Human; TAIFb Matched ELISA Antibody Pair Set, Human; TAIFc Matched ELISA Antibody Pair Set, Human; TAIFd Matched ELISA Antibody Pair Set, Human
IL-32 背景信息
IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.
全称
interleukin 32
参考文献
- Felaco P, et al. (2009) IL-32: a newly-discovered proinflammatory cytokine. J Biol Regul Homeost Agents. 23(3): 141-7.
- Kobayashi H, et al. (2009) Molecular characterization of IL-32 in human endothelial cells. Cytokine. 46(3): 351-8.
- Meyer N, et al. (2010) IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis. J Allergy Clin Immunol. 125(4): 858-865.
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