IL-4 Matched ELISA Antibody Pair Set,Human

For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
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IL-4 Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
IL-4 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human IL-4
线性范围
7.81-500 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 0.3 mg/mL of mouse anti-Human IL4 / IL-4 / Interleukin-4 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11846-MM05)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human IL4 / IL-4 / Interleukin-4 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.2 μg/mL in detection antibody dilution buffer before use. (Catalog: # 11846-MM04)
3. Standard Each vial contains 15 ng of recombinant Human IL4 / IL-4 / Interleukin-4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 500 pg/mL is recommended.
产品概述
This IL-4 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human IL-4 . It contains Human IL-4 capture antibody, Human IL-4 detector antibody (HRP) and a highly purified E. coli-expressed recombinant Human IL-4 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

IL-4 Matched ELISA Antibody Pair Set,Human 图片

IL-4 Matched ELISA Antibody Pair Set,Human: 别称

BCGF-1 Matched ELISA Antibody Pair Set, Human; BCGF1 Matched ELISA Antibody Pair Set, Human; BSF-1 Matched ELISA Antibody Pair Set, Human; BSF1 Matched ELISA Antibody Pair Set, Human; IL-4 Matched ELISA Antibody Pair Set, Human; Interleukin-4 Matched ELISA Antibody Pair Set, Human

IL-4 背景信息

Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
全称
interleukin 4
Related Pathways
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
参考文献
  • Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
  • Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
  • Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.
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