IL-6 Matched ELISA Antibody Pair Set,Human
The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.
IL-6 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
IL-6 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human IL-6
线性范围
2.34-150 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of mouse anti-Human IL-6 / Interleukin-6 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10395-MM14)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human IL-6 / Interleukin-6 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in Dilution Buffer before use. (Catalog: # 10395-MM72)
3. Standard Each vial contains 40 ng of recombinant Human IL-6 / Interleukin-6. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 150 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human IL-6 / Interleukin-6 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in Dilution Buffer before use. (Catalog: # 10395-MM72)
3. Standard Each vial contains 40 ng of recombinant Human IL-6 / Interleukin-6. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 150 pg/mL is recommended.
产品概述
This IL-6 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human IL-6 . It contains Human IL-6 capture antibody, Human IL-6 detector antibody
(HRP)
and a highly purified
E. coli-expressed
recombinant Human IL-6 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
IL-6 Matched ELISA Antibody Pair Set,Human 图片
IL-6 Matched ELISA Antibody Pair Set,Human: 别称
BSF2 Matched ELISA Antibody Pair Set, Human; HGF Matched ELISA Antibody Pair Set, Human; HSF Matched ELISA Antibody Pair Set, Human; IFNB2 Matched ELISA Antibody Pair Set, Human; IL-6 Matched ELISA Antibody Pair Set, Human; Interleukin-6 Matched ELISA Antibody Pair Set, Human
IL-6 背景信息
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.
全称
interleukin 6
Related Pathways
-
IL17 signaling pathway
参考文献
- Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
- Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
- Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
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Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF-κB signalling pathway by binding to eEF1A1AuthorWei, S;Wang, D;Li, H;Bi, L;Deng, J;Zhu, G;Zhang, J;Li, C;Li, M;Fang, Y;Zhang, G;Chen, J;Tao, S;Zhang, XE;Year2019JournalCell. Microbiol.
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