IL-8/CXCL8 Matched ELISA Antibody Pair Set,Human

For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
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IL-8/CXCL8 Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
IL-8/CXCL8 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human IL-8/CXCL8
线性范围
3.13-200 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 1 mg/mL of mouse anti-Human IL-8/Interleukin-8/CXCL8 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10098-MM05)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human IL-8/Interleukin-8/CXCL8 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in Dilution Buffer before use. (Catalog: # 10098-MM18)
3. Standard Each vial contains 20 ng of recombinant Human IL-8/Interleukin-8/CXCL8. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 200 pg/mL is recommended.
产品概述
This IL-8/CXCL8 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human IL-8/CXCL8 . It contains Human IL-8/CXCL8 capture antibody, Human IL-8/CXCL8 detector antibody (HRP) and a highly purified Yeast-expressed recombinant Human IL-8/CXCL8 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

IL-8/CXCL8 Matched ELISA Antibody Pair Set,Human 图片

IL-8/CXCL8 Matched ELISA Antibody Pair Set,Human: 别称

GCP-1 Matched ELISA Antibody Pair Set, Human; GCP1 Matched ELISA Antibody Pair Set, Human; IL-8 Matched ELISA Antibody Pair Set, Human; IL8 Matched ELISA Antibody Pair Set, Human; Interleukin-8 Matched ELISA Antibody Pair Set, Human; LECT Matched ELISA Antibody Pair Set, Human; LUCT Matched ELISA Antibody Pair Set, Human; LYNAP Matched ELISA Antibody Pair Set, Human; MDNCF Matched ELISA Antibody Pair Set, Human; MONAP Matched ELISA Antibody Pair Set, Human; NAF Matched ELISA Antibody Pair Set, Human; NAP-1 Matched ELISA Antibody Pair Set, Human; NAP1 Matched ELISA Antibody Pair Set, Human

IL-8/CXCL8 背景信息

Interleukin 8 (IL-8), also known as CXCL8, which is a chemokine with a defining CXC amino acid motif that was initially characterized for its leukocyte chemotactic activity, is now known to possess tumorigenic and proangiogenic properties as well. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL1, TNF, LPS, etc). In human gliomas, IL-8 is expressed and secreted at high levels both in vitro and in vivo, and recent experiments suggest it is critical to glial tumor neovascularity and progression. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Accumulating evidence has demonstrated that various types of cells can produce a large amount of IL-8/CXCL8 in response to a wide variety of stimuli, including proinflammatory cytokines, microbes and their products, and environmental changes such as hypoxia, reperfusion, and hyperoxia. Numerous observations have established IL-8/CXCL8 as a key mediator in neutrophil-mediated acute inflammation due to its potent actions on neutrophils. However, several lines of evidence indicate that IL-8/CXCL8 has a wide range of actions on various types of cells, including lymphocytes, monocytes, endothelial cells, and fibroblasts, besides neutrophils. The discovery of these biological functions suggests that IL-8/CXCL8 has crucial roles in various pathological conditions such as chronic inflammation and cancer. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.
全称
chemokine (C-X-C motif) ligand 8
研究领域
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Mukaida N. (2003) Pathophysiological roles of interleukin-8/CXCL8 in pulmonary diseases. Am J Physiol Lung Cell Mol Physiol. 284(4): L566-77.
  • Brat DJ, et al. (2005) The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis. Neuro Oncol. 7(2): 122-33.
  • Bendrik C, et al. (2009) Estradiol increases IL-8 secretion of normal human breast tissue and breast cancer in vivo. J Immunol. 182(1): 371-8.
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