Intrinsic Factor Matched ELISA Antibody Pair Set,Human

For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
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Intrinsic Factor Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
Intrinsic Factor Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human Intrinsic Factor
线性范围
15.63-1000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 1.5 mg/mL of rabbit anti-Human Gastric intrinsic factor / GIF monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 13544-R016)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human Gastric intrinsic factor / GIF polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 36 ng of recombinant Human Gastric intrinsic factor / GIF. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended.
产品概述
This Intrinsic Factor Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Intrinsic Factor . It contains Human Intrinsic Factor capture antibody, Human Intrinsic Factor detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human Intrinsic Factor protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Intrinsic Factor Matched ELISA Antibody Pair Set,Human 图片

Intrinsic Factor Matched ELISA Antibody Pair Set,Human: 别称

IF Matched ELISA Antibody Pair Set, Human; IFMH Matched ELISA Antibody Pair Set, Human; INF Matched ELISA Antibody Pair Set, Human; TCN3 Matched ELISA Antibody Pair Set, Human

Intrinsic Factor 背景信息

Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.
全称
gastric intrinsic factor (vitamin B synthesis)
参考文献
  • Gerdin AK. (2010) The Sanger Mouse Genetics Programme: high throughput characterisation of knockout mice. Acta Opthalmologica. 88:925-7.
  • AU - Berlin H, et al. (1968) ORAL TREATMENT OF PERNICIOUS ANEMIA WITH HIGH DOSES OF VITAMIN B12 WITHOUT INTRINSIC FACTOR. Acta Medica Scandinavica. 184(1-6):247-58.
  • Hewitt JE, et al. (1991) Human gastric intrinsic factor: characterization of cDNA and genomic clones and localization to human chromosome 11. Genomics. 10(2):432-40.
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