1. Capture Antibody 0.5 mg/mL of mouse anti-Human MICB / MIC-B monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 0.5 μg/mL in PBS before coating. (Catalog: # 10759-MM04) 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human MICB / MIC-B polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard Each vial contains 124 ng of recombinant Human MICB / MIC-B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
This MICB Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human MICB . It contains Human MICB capture antibody, Human MICB detector antibody
and a highly purified
recombinant Human MICB protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
MICB Matched ELISA Antibody Pair Set,Human 图片
MICB Matched ELISA Antibody Pair Set,Human: 别称
PERB11.2 Matched ELISA Antibody Pair Set, Human
MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.
MHC class I polypeptide-related sequence B
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