PBK/TOPK Matched ELISA Antibody Pair Set,Human

For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
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PBK/TOPK Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
PBK/TOPK Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human PBK/TOPK
线性范围
62.5-4000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 0.3 mg/mL of mouse anti-PBK monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10729-MM10)
2. Detection Antibody 0.5 mg/mL mouse-anti-PBK monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10729-MM05)
3. Standard Each vial contains 40 ng of recombinant PBK. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4 ng/mL is recommended.
产品概述
This PBK/TOPK Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human PBK/TOPK . It contains Human PBK/TOPK capture antibody, Human PBK/TOPK detector antibody (HRP) and a highly purified Baculovirus-Insect-expressed recombinant Human PBK/TOPK protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

PBK/TOPK Matched ELISA Antibody Pair Set,Human 图片

PBK/TOPK Matched ELISA Antibody Pair Set,Human: 别称

CT84 Matched ELISA Antibody Pair Set, Human; HEL164 Matched ELISA Antibody Pair Set, Human; Nori-3 Matched ELISA Antibody Pair Set, Human; SPK Matched ELISA Antibody Pair Set, Human; TOPK Matched ELISA Antibody Pair Set, Human

PBK/TOPK 背景信息

PDZ binding kinase (PBK), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family, and has all the characteristic protein kinase subdomains and a C-terminal PDZ-binding T/SXV motif. PBK is expressed in the testis restrictedly expressed in outer cell layer of seminiferous tubules, as well as placenta. PBK may be enrolled in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. This mitotic kinase phosphorylates MAP kinase p38 and seems to be active in mitosis. When phosphorylated, PBK forms a protein-protein interaction with tumor suppressor p53 (TP53), leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. The expression level of PBK is thus upregulated in a variety of neoplasms including hematological malignancies.
全称
PDZ binding kinase
参考文献
  • Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.
  • Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.
  • Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
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