PDGFRA Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
Cat: SEK10556
PDGFRA Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
PDGFRA Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human PDGFRA
线性范围
31.25-2000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of mouse anti-PDGFRA monoclonal antibody. Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 10556-MM07)
2. Detection Antibody 0.4 mg/mL rabbit anti-PDGFRA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 35 ng of recombinant PDGFRA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.
2. Detection Antibody 0.4 mg/mL rabbit anti-PDGFRA polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 35 ng of recombinant PDGFRA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2 ng/mL is recommended.
产品概述
This PDGFRA Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human PDGFRA . It contains Human PDGFRA capture antibody, Human PDGFRA detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human PDGFRA protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
PDGFRA Matched ELISA Antibody Pair Set,Human 图片
PDGFRA Matched ELISA Antibody Pair Set,Human: 别称
CD140A Matched ELISA Antibody Pair Set, Human; PDGFR-2 Matched ELISA Antibody Pair Set, Human; PDGFR2 Matched ELISA Antibody Pair Set, Human; RHEPDGFRA Matched ELISA Antibody Pair Set, Human
PDGFRA 背景信息
PDGFRA, also known as CD14a, together with the structurally homolog protein PDGFRB (CD14b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development. Additionally, PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC). PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas.
全称
platelet-derived growth factor receptor, alpha polypeptide
参考文献
- Oseini AM, et al. (2009) PDGFRalpha: a new therapeutic target in the treatment of hepatocellular carcinoma? Expert Opin Ther Targets. 13(4): 443-54.
- Meister M, et al. (2009) Expression and mutational status of PDGFR in thymic tumours. Anticancer Res. 29(10): 4057-61.
- Martinho O, et al. (2009) Expression, mutation and copy number analysis of platelet-derived growth factor receptor A (PDGFRA) and its ligand PDGFA in gliomas. Br J Cancer. 101(6): 973-82.
