PLA2G1B Matched ELISA Antibody Pair Set,Mouse
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
PLA2G1B Matched ELISA Antibody Pair Set,Mouse 产品信息
产品名称
PLA2G1B Matched ELISA Antibody Pair Set,Mouse
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Mouse PLA2G1B
线性范围
3.125-200 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse PLA2G1B monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50329-R001)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse PLA2G1B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50329-R004)
3. Standard Each vial contains 12 ng of recombinant Mouse PLA2G1B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 200 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse PLA2G1B monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50329-R004)
3. Standard Each vial contains 12 ng of recombinant Mouse PLA2G1B. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 200 pg/mL is recommended.
产品概述
This PLA2G1B Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse PLA2G1B . It contains Mouse PLA2G1B capture antibody, Mouse PLA2G1B detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Mouse PLA2G1B protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
PLA2G1B Matched ELISA Antibody Pair Set,Mouse 图片
PLA2G1B Matched ELISA Antibody Pair Set,Mouse: 别称
Pla2a Matched ELISA Antibody Pair Set, Mouse; sPLA2IB Matched ELISA Antibody Pair Set, Mouse
PLA2G1B 背景信息
phospholipase A2, also known as Phosphatidylcholine 2-acylhydrolase 1B, Group IB phospholipase A2, PLA2 and PLA2G1B, is a secreted protein that belongs to the phospholipase A2 family. Phospholipase A2 / PLA2G1B catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic Phospholipase A2 / PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of Phospholipase A2 / PLA2G1B have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets. Mice lacking in Phospholipase A2 / PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.
全称
phospholipase A2, group IB (pancreas)
参考文献
- Labonté,E.D. et al., 2006, Diabetes. 55 (4) :935-41.
- Mounier,C.M. et al.,2008, Br J Cancer. 98 (3):587-95.
- Hui,D.Y. et al., 2009, Br J Pharmacol. 157 (7):1263-9.
- Labonté,E.D. et al., 2010, FASEB J. 24 (7):2516-24.
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