PVRL1/NECTIN1 Matched ELISA Antibody Pair Set,Rat

For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
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PVRL1/NECTIN1 Matched ELISA Antibody Pair Set,Rat 产品信息

产品名称
PVRL1/NECTIN1 Matched ELISA Antibody Pair Set,Rat
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Rat PVRL1/NECTIN1
线性范围
23.45-1500 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 2 mg/mL of rabbit anti-Rat CD111 / Nectin-1 / PVRL1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 80244-R029)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Rat CD111 / Nectin-1 / PVRL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 80244-R004)
3. Standard Each vial contains 105 ng of recombinant Rat CD111 / Nectin-1 / PVRL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.
产品概述
This PVRL1/NECTIN1 Matched ELISA Antibody Pair Set,Rat is a solid phase sandwich ELISA for quantitative determination of Rat PVRL1/NECTIN1 . It contains Rat PVRL1/NECTIN1 capture antibody, Rat PVRL1/NECTIN1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Rat PVRL1/NECTIN1 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

PVRL1/NECTIN1 Matched ELISA Antibody Pair Set,Rat 图片

PVRL1/NECTIN1 背景信息

Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.
全称
poliovirus receptor-related 1 (herpesvirus entry mediator C)
参考文献
  • Yoon M, et al. (2002) Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 76(14): 7203-8.
  • Mata M, et al. (2001) HveC (nectin-1) is expressed at high levels in sensory neurons, but not in motor neurons, of the rat peripheral nervous system. J Neurovirol. 7(5): 476-80.
  • Haarr L, et al. (2001) Transcription from the gene encoding the herpesvirus entry receptor nectin-1 (HveC) in nervous tissue of adult mouse. Virology. 287(2): 301-9.
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