S100A1 Matched ELISA Antibody Pair Set,Mouse

For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
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S100A1 Matched ELISA Antibody Pair Set,Mouse 产品信息

产品名称
S100A1 Matched ELISA Antibody Pair Set,Mouse
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Mouse S100A1
线性范围
31.25-2000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 0.5 mg/mL of rabbit anti-Mouse S100A1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50266-R049)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse S100A1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 50266-R148)
3. Standard Each vial contains 190 ng of recombinant Mouse S100A1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
产品概述
This S100A1 Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse S100A1 . It contains Mouse S100A1 capture antibody, Mouse S100A1 detector antibody (HRP) and a highly purified E. coli-expressed recombinant Mouse S100A1 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

S100A1 Matched ELISA Antibody Pair Set,Mouse 图片

S100A1 Matched ELISA Antibody Pair Set,Mouse: 别称

AI266795 Matched ELISA Antibody Pair Set, Mouse; S100 Matched ELISA Antibody Pair Set, Mouse; S100a Matched ELISA Antibody Pair Set, Mouse

S100A1 背景信息

S100A1 is a Ca2+ binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.
全称
S100 calcium binding protein A1
参考文献
  • Remppis, A .et al., 1996, Biochim. Biophs. Acta. 1313: 253-257.
  • Most, P. et al., 2001, Proc. Natl. Acad. Sci. U.S.A. 98: 13889-13894
  • Okada, M. et al., 2004, J. Biol. Chem. 279: 4221-4233.
  • Schafer, W.E. et al., 1995, Genomics. 25: 638-643.
  • Landar, A. et al., 1996, Cell. Calcium. 20: 279-285.
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