SPARCL1 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
SPARCL1 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
SPARCL1 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human SPARCL1
线性范围
93.75-6000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1.0 mg/mL of mouse anti-SPARCL1 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 10046-MM01)
2. Detection Antibody 0.5 mg/mL mouse anti-SPARCL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10046-MM05)
3. Standard Each vial contains 160 ng of recombinant SPARCL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 6000 pg/mL is recommended.
2. Detection Antibody 0.5 mg/mL mouse anti-SPARCL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10046-MM05)
3. Standard Each vial contains 160 ng of recombinant SPARCL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 6000 pg/mL is recommended.
产品概述
This SPARCL1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human SPARCL1 . It contains Human SPARCL1 capture antibody, Human SPARCL1 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human SPARCL1 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
SPARCL1 Matched ELISA Antibody Pair Set,Human 图片
SPARCL1 Matched ELISA Antibody Pair Set,Human: 别称
MAST 9 Matched ELISA Antibody Pair Set, Human; MAST9 Matched ELISA Antibody Pair Set, Human; PIG33 Matched ELISA Antibody Pair Set, Human; SC1 Matched ELISA Antibody Pair Set, Human; SPARCL1 Matched ELISA Antibody Pair Set, Human
SPARCL1 背景信息
SPARC-like protein 1 (SPARCL1; also known as SC1, high endothelial venule protein, or hevin) is an extracellular matrix-associated, secreted glycoprotein belonging to the secreted protein acidic and rich in cysteine (SPARC) family of matricellular proteins. It contains three conserved structural domains that are implicated in the regulation of cell adhesion, migration, and proliferation. SPARCL1 is expressed during embryogenesis and tissue remodeling and is especially prominent in brain and vasculature. Its down-regulation in a number of cancers and the possibility of its functional compensation by SPARC has led to recent interest in hevin as a tumor suppressor and regulator of angiogenesis. SPARCL1 has antiadhesive properties, and loss of SPARCL1 expression is associated with increased proliferative activity and cell cycle progression. It is suggested that it may influence multiple cellular processes during distinct stages of brain development and function. Besides, SPARCL1 can influence the function of astroglial cells in the developing and mature central nervous system (CNS).
全称
SPARC like 1
参考文献
- Sullivan MM, et al. (2004) Hevin/SC1, a matricellular glycoprotein and potential tumor-suppressor of the SPARC/BM-40/Osteonectin family. Int J Biochem Cell Biol. 36(6): 991-6.
- Esposito I, et al. (2007) Tumor-suppressor function of SPARC-like protein 1/Hevin in pancreatic cancer. Neoplasia. 9(1): 8-17.
- Weimer JM, et al. (2008) A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system. Glia. 56(9): 935-41.
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