ST6GAL1 Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
ST6GAL1 Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
ST6GAL1 Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human ST6GAL1
线性范围
78.13-5000 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of rabbit anti-Human ST6GAL1/ST6GAL-I monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 11590-R001)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human ST6GAL1/ST6GAL-I polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 83 ng of recombinant Human ST6GAL1/ST6GAL-I. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human ST6GAL1/ST6GAL-I polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 83 ng of recombinant Human ST6GAL1/ST6GAL-I. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 5000 pg/mL is recommended.
产品概述
This ST6GAL1 Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human ST6GAL1 . It contains Human ST6GAL1 capture antibody, Human ST6GAL1 detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human ST6GAL1 protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
ST6GAL1 Matched ELISA Antibody Pair Set,Human 图片
ST6GAL1 Matched ELISA Antibody Pair Set,Human: 别称
SIAT1 Matched ELISA Antibody Pair Set, Human; ST6GalI Matched ELISA Antibody Pair Set, Human; ST6N Matched ELISA Antibody Pair Set, Human
ST6GAL1 背景信息
Beta-galactoside alpha-2,6-sialyltransferase 1, also known as B-cell antigen CD75, Sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6GAL1 and SIAT1, is a single-pass type II membrane protein that belongs to the glycosyltransferase 29 family. Sialyltransferases are key enzymes in the biosynthesis of sialoglycoconjugates that catalyze the transfer of sialic residue from its activated form to an oligosaccharidic acceptor. ST6GAL1 / SIAT1 is normally found in the Golgi but can be proteolytically processed to a soluble form. It is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. β-Galactoside α2,6-sialyltransferases ST6GAL1 and ST6GAL2 are the two unique members of the ST6GAL family described in higher vertebrates. ST6GAL1 / SIAT1 transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
全称
ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
参考文献
- Collins,B.E. et al., 2006, Nat Immunol. 7(2):199-206.
- Videira,P.A. et al., 2008, Glycoconj J. 25(3): 259-68.
- Petit,D. et al., 2010, J Biol Chem. 285(49): 38399-414.
- Kroes,R.A. et al., 2010, Proc Natl Acad Sci USA.107(28):12646-51.
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