TNF-alpha Matched ELISA Antibody Pair Set,Mouse
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
TNF-alpha Matched ELISA Antibody Pair Set,Mouse 产品信息
产品名称
TNF-alpha Matched ELISA Antibody Pair Set,Mouse
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Mouse TNF-alpha
线性范围
28.3-1800 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50349-R023)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.35 μg/mL in Dilution Buffer before use.
3. Standard Each vial contains 92 ng of recombinant Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 1800 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.35 μg/mL in Dilution Buffer before use.
3. Standard Each vial contains 92 ng of recombinant Mouse TNF-alpha / TNFA / TNFSF2 ELISA Pair Set. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 1800 pg/mL is recommended.
产品概述
This TNF-alpha Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse TNF-alpha . It contains Mouse TNF-alpha capture antibody, Mouse TNF-alpha detector antibody
(HRP)
and a highly purified
E. coli-expressed
recombinant Mouse TNF-alpha protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
TNF-alpha Matched ELISA Antibody Pair Set,Mouse 图片
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TNF-alpha Matched ELISA Antibody Pair Set,Mouse: 别称
DIF Matched ELISA Antibody Pair Set, Mouse; TNF-a Matched ELISA Antibody Pair Set, Mouse; TNF-alpha Matched ELISA Antibody Pair Set, Mouse; Tnfa Matched ELISA Antibody Pair Set, Mouse; TNFalpha Matched ELISA Antibody Pair Set, Mouse; Tnfsf1a Matched ELISA Antibody Pair Set, Mouse; TNFSF2 Matched ELISA Antibody Pair Set, Mouse
TNF-alpha 背景信息
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
TNF-alpha Protein & Antibody
全称
tumor necrosis factor
Related Pathways
-
Death Receptor Signaling -
NF-kB (NFkB) Pathway -
IL17 signaling pathway
参考文献
- Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
- Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
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The effect of exposure time and concentration of airborne PM2.5 on lung injury in mice: A transcriptome analysisAuthorWang, H;Shen, X;Liu, J;Wu, C;Gao, J;Zhang, Z;Zhang, F;Ding, W;Lu, Z;Year2019JournalRedox BiologyApplicationELISA
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