TrkB Matched ELISA Antibody Pair Set,Human
For the orders from outside of China: MOQ 15 plates, lead time: 2 weeks.
TrkB Matched ELISA Antibody Pair Set,Human 产品信息
产品名称
TrkB Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human TrkB
线性范围
10.98-700 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 1 mg/mL of mouse anti-Human TrkB/NTRK2 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10047-MM12)
2. Detection Antibody 0.2 mg/mL of mouse anti-Human TrkB/NTRK2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.3 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10047-MM01)
3. Standard Each vial contains 28 ng of recombinant Human TrkB/NTRK2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 700 pg/mL is recommended.
2. Detection Antibody 0.2 mg/mL of mouse anti-Human TrkB/NTRK2 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.3 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10047-MM01)
3. Standard Each vial contains 28 ng of recombinant Human TrkB/NTRK2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 700 pg/mL is recommended.
产品概述
This TrkB Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human TrkB . It contains Human TrkB capture antibody, Human TrkB detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Human TrkB protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
TrkB Matched ELISA Antibody Pair Set,Human 图片
TrkB Matched ELISA Antibody Pair Set,Human: 别称
GP145-TrkB Matched ELISA Antibody Pair Set, Human; GP145-TrkB/GP95-TrkB Matched ELISA Antibody Pair Set, Human; Tkrb Matched ELISA Antibody Pair Set, Human; trk-B Matched ELISA Antibody Pair Set, Human; TRKB Matched ELISA Antibody Pair Set, Human
TrkB 背景信息
TrkB receptor also known as TrkB tyrosine kinase or BDNF/NT-3 growth factors receptor or neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) is a single transmembrane catalytic receptor with intracellular tyrosine kinase activity. TrkB/NTRK2 is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. TrkB tyrosine kinase (TrkB) or NTRK2 is coupled to the Ras, Cdc42/Rac/RhoG, MAPK, PI3-K, and PLCgamma signaling pathways. There are four members of the Trk family; TrkA, TrkB, and TrkC and a related p75NTR receptor. Each family member binds different neurotrophins with varying affinities. TrkB/NTRK has the highest affinity for brain-derived neurotrophic factor (BDNF) and is involved in neuronal plasticity, long-term potentiation, and apoptosis of CNS neurons. Other neurotrophins include nerve growth factor(NGF), neurotrophin-3 and neurotrophin-4. TrkB/NTRK is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signaling through this kinase leads to cell differentiation. Mutations in TrkB/NTRK have been associated with obesity and mood disorders.
全称
neurotrophic tyrosine kinase, receptor, type 2
参考文献
- Klein R, et al. (1990) The trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain. Cell. 61 (4): 647-56.
- Rose CR, et al. (2003) Truncated TrkB-T1 mediates neurotrophin-evoked calcium signalling in glia cells. Nature. 426 (6962): 74-8.
- Yamada K, et al. (2004) Brain-derived neurotrophic factor/TrkB signaling in memory processes. J Pharmacol Sci. 91 (4): 267-70.
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Mature brain-derived neurotrophic factor and its receptor TrkB are upregulated in human glioma tissuesAuthorXiong, J;Zhou, L;Lim, Y;Yang, M;Zhu, YH;Year2015JournalOncology LettersApplicationELISA for mature TrkB in tissue samples
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ProBDNF/p75NTR/sortilin pathway is activated in peripheral blood of patients with alcohol dependenceAuthorZhou, L;Xiong, J;Ruan, CS;Ruan, Y;Liu, D;Bao, JJ;Zhou, XF;Year2018JournalTransl PsychiatryApplicationELISA (serum)
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