Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set,Human

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Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set,Human 产品信息

产品名称
Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set,Human
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Human Von Willebrand Factor/vWF
线性范围
109.38-7000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 1 mg/mL of mouse anti-Human VWF monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10973-MM05)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human VWF monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10973-R111)
3. Standard Each vial contains 180 ng of recombinant Human VWF. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 7000 pg/mL is recommended.
产品概述
This Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human Von Willebrand Factor/vWF . It contains Human Von Willebrand Factor/vWF capture antibody, Human Von Willebrand Factor/vWF detector antibody (HRP) and a highly purified CHO Stable-expressed recombinant Human Von Willebrand Factor/vWF protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set,Human 图片

Von Willebrand Factor/vWF Matched ELISA Antibody Pair Set,Human: 别称

F8VWF Matched ELISA Antibody Pair Set, Human; Von Willebrand Factor Matched ELISA Antibody Pair Set, Human; VWD Matched ELISA Antibody Pair Set, Human

Von Willebrand Factor/vWF 背景信息

Von Willebrand Factor (VWF) is a multimeric glycoprotein involved in hemostasis in blood, binds receptors on the surface of platelets and in connective tissue, thereby mediating the adhesion of platelets to sites of vascular injury. From studies it appears that VWF protein uncoils under these circumstances, decelerating passing platelets. VWF protein is deficient or defective in von Willebrand disease (VWD) and is involved in a large number of other diseases, including thrombosis, thrombotic thrombocytopenic purpura, Stroke, Heyde's syndrome, possibly hemolytic-uremic syndrome and so on.
全称
von Willebrand factor
参考文献
  • Sadler JE. (1998) Biochemistry and genetics of von Willebrand factor. Annu Rev Biochem, 67: 395-424.
  • Batlle J, et al. (2009) Von Willebrand factor/factor VIII concentrates in the treatment of von Willebrand disease. Blood Coagul Fibrinolysis. 20(2):89-100.
  • Sadler JE. (2009) von Willebrand factor assembly and secretion. J Thromb Haemost. 7 Suppl 1:24-7.
  • Auton M, et al. (2010) The mechanism of VWF-mediated platelet GPIbalpha binding. Biophys J. 99(4):1192-201.
  • Bowen DJ. (2010) Sugar targets VWF for the chop. Blood. 115(13):2565.
  • Lpez JA, et al. (2010) VWF self-association: more bands for the buck. Blood. 116(19):3693-4.
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