Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set

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Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set 产品信息

产品名称
Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of ZIKV ZIKV Envelope
线性范围
125-8000 pg/mL
ELISA 抗体对套装(非即用型)组分
1. Capture Antibody 0.5 mg/mL of mouse anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 40543-MM35)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use. (Catalog: # 40543-R033)
3. Standard Each vial contains 510 ng of recombinant Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E). Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 8000 pg/mL is recommended.
产品概述
This Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set is a solid phase sandwich ELISA for quantitative determination of ZIKV ZIKV Envelope . It contains ZIKV ZIKV Envelope capture antibody, ZIKV ZIKV Envelope detector antibody (HRP) and a highly purified HEK293-expressed recombinant ZIKV ZIKV Envelope protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Zika virus (strain Zika SPH2015) Envelope Protein (ZIKV-E) ELISA Pair Set 图片

ZIKV Envelope 背景信息

Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.
参考文献
  • Marina Basarab specialty registrar. et al .Zika virus. BMJ 2016; 352:i1049 doi: 10.1136/bmj.i1049
  • Pinto Júnior VL, et al. Zika virus: a review to clinicians, Acta Med Port 2015 Nov-Dec;28(6):760-765
  • L Zammarchi, et al.Zika virus infection in a traveler returning to Europe from Brazil, March 2015
  • Arturo Galindo-Fraga, et al. Zika virus: A new epidemic on our doorstep. Rev Inves Clin.2015, 67: 329-32
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