PD1 / PDCD1 / CD279 Blocking Antibody

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PD1 / PDCD1 / CD279 Blocking Antibody (Mouse Monoclonal 抗体) 产品信息

产品名称
PD1 / PDCD1 / CD279 Blocking Antibody
经验证的应用
Neutralization
交叉反应
Reacts with: Human
特异性
Human PD-1
No cross-reactivity with mouse PD1 (Catalog # 50124-M08H) Protein in ELISA assay
免疫原
Recombinant Human PD1 / PDCD1 / CD279 protein (Catalog#10377-H08H)
制备方法
It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant Human PD1 / PDCD1 / CD279. The antibody was produced using recombinant antibody technology.
来源
Monoclonal mouse (varialbe region) / human (kappa / IgG1 constant) chimeric antibody Clone #mhT28
纯化
Protein A
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
内毒素
Please contact us for more information.
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

PD1 / PDCD1 / CD279 Blocking Antibody (Mouse Monoclonal 抗体) 经验证的应用

Notes
Block: In a functional ELISA which immobilized recombinant Human PD-L1 (Catalog # 10084-H02H) at 10 μg/mL (100 µL/well) in the plate, the Mouse and Human Chimeric anti-Human PD1 Monoclonal Antibody (Catalog # 10377-mhT28) can block the binding of 0.2 μg/mL of recombinant Human PD1/Fc Chimera (Catalog # 10377-H03H) to human PD-L1,the EC50 is 0.23 μg/mL.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

PD1 / PDCD1 / CD279 Blocking Antibody: 别称

Anti-CD279 Antibody; Anti-hPD-1 Antibody; Anti-hPD-l Antibody; Anti-hSLE1 Antibody; Anti-PD-1 Antibody; Anti-PD1 Antibody; Anti-SLEB2 Antibody

PD-1 背景信息

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-1 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.
全称
programmed cell death 1
参考文献
  • James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.
  • Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.
  • del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.
  • Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.
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