Protein Refolding Service / Protein Production Service in E. coli.

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Protein Refolding Service / Protein Production Service in E. coli.

Full services: From gene cloning/synthesis to purified proteins
Host Cells: Bacterial E. Coli. (Escherichia coli) Cells
Technology: Inclusion body production + protein refolding
Purification: Typically expressed as native protein without a tag. If necessary, small
tags such as 6X his and Flag can be used.
Deliverable: Purified recombinant proteins (quantities to be specified by customer)
Quality Specification: typically >90% purity by SDS page
Typical Timeline: 10-15 weeks

Track Records & Key Facts

• > 300 Staff
• > 80 Bioreactors
• > 30 Purification System
• > 10,000 Mab Production
• > 5000 Protein Bulks
• > 5000 antibody development
• 1-100mg Mab Production in 3-6 weeks
• 1-10 g Mab Production in 6-8 weeks
• Preferred CRO Partner for Multiple Top-10 Pharmas

Protein Refolding Service Description

In E. coli. expression system, there are still many proteins that can only be expressed as inclusion body and have to be refolded into soluble and function forms. Protein refolding is an art work that needs lots of experience and hard work.

Sino Biological has devoted significant resources in development of protein expression and production in E. coli. system. We have developed in house a refolding buffer screening system that allows us to quickly identify suitable buffers for refolding for a particular protein. In the past couple of years (2008-2014), we have successfully produced over 1000 recombinant proteins using our E. coli. expression system. Many of these proteins are tough ones that cannot ...... < Learn More>

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