Human C5 qPCR Primer Pair

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Human C5 qPCR Primer Pair: 基本信息

基因信息
种属:
Human
产品信息
寡核苷酸类型:
qPCR Primers
组分:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
qPCR 引物描述:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
应用 & 质控
应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
运输方式:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存条件:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

特色与优势

独特的引物设计

在特定基因的不同变体保守区内设计引物,该对引物至少有一条引物跨越内含子或产物跨越内含子,有效避免基因组 DNA 的扩增。

严格的筛选验证过程

用质粒标准品对 qPCR 引物的灵敏度、扩增效率和特异性进行筛选,用阳性组织或细胞验证确认。

统一的 PCR 条件,操作方便,节约时间与成本

~100% 的扩增效率,保证 RNA 定量的准确性

Human C5 qPCR Primer Pair: 别称

C5a qPCR Primer Pairs, Human; C5b qPCR Primer Pairs, Human; C5D qPCR Primer Pairs, Human; Complement C5 qPCR Primer Pairs, Human; CPAMD4 qPCR Primer Pairs, Human; ECLZB qPCR Primer Pairs, Human

C5 背景信息

C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.
全称
complement component 5
Related Pathways
  • Complement Activation Pathways
    Complement Activation Pathways
参考文献
  • Guo RF, et al.. (2005) Role of C5a in inflammatory responses. Annu Rev Immunol. 23: 821-52.
  • Guo RF, et al. (2006) C5a, a therapeutic target in sepsis. Recent Pat Antiinfect Drug Discov. 1(1): 57-65.
  • Manthey HD, et al. (2009) Complement component 5a (C5a). Int J Biochem Cell Biol. 41(11): 2114-7.

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