The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
Lyophilized qPCR primer mix is shipped at ambiente temperatura
The lyophilized product is stable for one year from date of receipt when stored at -20℃.
The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***
在特定基因的不同变体保守区内设计引物，该对引物至少有一条引物跨越内含子或产物跨越内含子，有效避免基因组 DNA 的扩增。
用质粒标准品对 qPCR 引物的灵敏度、扩增效率和特异性进行筛选，用阳性组织或细胞验证确认。
统一的 PCR 条件，操作方便，节约时间与成本
~100% 的扩增效率，保证 RNA 定量的准确性
Human MMP13 qPCR Primer Pair: 别称
CLG3 qPCR Primer Pairs, Human; MANDP1 qPCR Primer Pairs, Human; MMP-13 qPCR Primer Pairs, Human
Matrix metalloproteinases (MMPs) such as MMP13 promote tumor growth and progression by mediating extracellular matrix (ECM) reorganization and regulating the biological activity of cytokines. Matrix metalloproteinase 13 (MMP13) plays a central role in the MMP activation cascade that enables degradation of the extracellular matrix and basement membranes, and it is identified as a potential driver in oral carcinogenesis.MMP13 is enriched in mature chondrocytes and is considered a prime cause of ECM degradation in the osteoarthritic articular cartilage in temporomandibular joints. A missense mutation of MMP13 causes the Missouri type of human Spondyloepimetaphyseal dysplasia (SEMD(MO)), an autosomal dominant disorder characterized by defective growth and modeling of vertebrae and long bones.