Mouse LIF qPCR Primer Pair

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Mouse LIF qPCR Primer Pair: 基本信息

基因信息
种属:
Mouse
产品信息
寡核苷酸类型:
qPCR Primers
组分:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
qPCR 引物描述:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
应用 & 质控
应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
运输方式:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存条件:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

特色与优势

独特的引物设计

在特定基因的不同变体保守区内设计引物,该对引物至少有一条引物跨越内含子或产物跨越内含子,有效避免基因组 DNA 的扩增。

严格的筛选验证过程

用质粒标准品对 qPCR 引物的灵敏度、扩增效率和特异性进行筛选,用阳性组织或细胞验证确认。

统一的 PCR 条件,操作方便,节约时间与成本

~100% 的扩增效率,保证 RNA 定量的准确性

LIF 背景信息

Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It is involved in growth promotion and cell differentiation of different types of target cells, influence bone metabolism, cachexia, neural development, embryogenesis, and inflammation. LIF has potent proinflammatory properties, being the inducer of the acute phase protein synthesis and affecting cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic, and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal-transducing subunit. This leads to the activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origins.
全称
leukemia inhibitory factor
参考文献
  • Salas EM, et al. (2011) LIF, a Novel STAT5-Regulated Gene, Is Aberrantly Expressed in Myeloproliferative Neoplasms. Genes Cancer. 2 (5): 593-6.
  • Chodorowska G, et al. (2004) Leukemia inhibitory factor (LIF) and its biological activity. Ann Univ Mariae Curie Sklodowska Med. 59 (2): 189-93.
  • Garcia-Campana AM, et al. (2007) LIF detection of peptides and proteins in CE. Electrophoresis. 28 (1-2): 208-32.
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