Rat AKR1A1 qPCR Primer Pair

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Rat AKR1A1 qPCR Primer Pair: 基本信息

基因信息
种属:
Rat
产品信息
寡核苷酸类型:
qPCR Primers
组分:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
qPCR 引物描述:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
应用 & 质控
应用:
SYBR® Green-based quantitative real-time PCR (qPCR).
质控:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
储存 & 运输
运输方式:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
储存条件:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

特色与优势

独特的引物设计

在特定基因的不同变体保守区内设计引物,该对引物至少有一条引物跨越内含子或产物跨越内含子,有效避免基因组 DNA 的扩增。

严格的筛选验证过程

用质粒标准品对 qPCR 引物的灵敏度、扩增效率和特异性进行筛选,用阳性组织或细胞验证确认。

统一的 PCR 条件,操作方便,节约时间与成本

~100% 的扩增效率,保证 RNA 定量的准确性

Rat AKR1A1 qPCR Primer Pair: 别称

Akr1a4 qPCR Primer Pairs, Rat

AKR1A1 背景信息

Aldehyde reductase (AKR1A1) is a member of the aldo-keto reductase superfamily, which consists of more than 40 known enzymes and proteins that includes variety of monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase. Aldehyde reductase has wide substrate specificities for carbonyl compounds. These enzymes are implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Aldehyde reductase possess a structure with a beta-alpha-beta fold which contains a novel NADP-binding motif. The binding site is located in a large, deep, elliptical pocket in the C-terminal end of the beta sheet, the substrate being bound in an extended conformation. This binding is more similar to FAD- than to NAD(P)-binding oxidoreductases. AKR1A1 is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.
全称
aldo-keto reductase family 1, member A1 (aldehyde reductase)
参考文献
  • Bohren KM, et al. (1989) The aldo-keto reductase superfamily. cDNAs and deduced amino acid sequences of human aldehyde and aldose reductases. J Biol Chem. 264 (16): 9547-51.
  • Fujii J, et al. (1999) The structural organization of the human aldehyde reductase gene, AKR1A1, and mapping to chromosome. Cytogenetics and Cell Genetics . 84 (3-4): 33-2.
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