Qualitative Analysis Service of Gene Expression by qPCR

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Qualitative Analysis using qPCR

Real-time quantitative PCR is aslo used for gene expression detection and qualitative analysis.The SYBR Green method usually appropriate.A melting curves shows if there is a target gene or a DNA fragment in the detected samples by performing a qPCR.

The main applications qualitative analysis by qPCR include:
• Virus and pathogen detection
• GMO detection
• Cultivar identification
• Mutant genotyping
• SNP analysis

Amplification curve and melting curve of target gene

Example

qPCR identification of humanized IL6 transgenic mice
Sample description:
Humanized IL6 transgenic mice are identified by qPCR. If the IL6 gene in transgenic mice are replaced with human IL6 gene, the experiment succeed.
Sample names: IL6 transgenic mouse、Wild-Type mouse
Detected genes: human IL6、mouse IL6、mouse gapdh(reference gene)
Detection method:
SYBR Green qPCR method
Test experiment report:
1. Amplification results of human IL6、mouse IL6、mouse gapdh in transgenic mice and wild-type mice.

Amplification curves of human IL6、mouse IL6、mouse gapdh

Melting curves of human IL6、mouse IL6、mouse gapdh

2. Electrophoresis results of human IL6、mouse IL6、mouse gapdh in transgenic mice and wild-type mice.

Electrophoresis results in 2% agarose

3. Mouse IL6 genes have been replaced with human IL6 genes in humanized IL6 transgenic mice.

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Real-time PCR Service: Gene Expression Analysis by qPCR+
- SYBR Green qPCR Method
- Taqman probe qPCR Method
- Qualitative Analysis Service of Gene Expression by qPCR
- Relative Quantitative Analysis Service of Gene Expression by qPCR
- Absolute Quantitative Analysis Service of Gene Expression by qPCR
- SNP Analysis Service by qPCR
- qPCR service request