|qPCR Master Mix||10000+qPCR primers||10000+qPCR standards|
High amplification efficiency
Wide linear range
Real-time quantitative PCR is aslo used for gene expression detection and qualitative analysis.The SYBR Green method usually appropriate.A melting curves shows if there is a target gene or a DNA fragment in the detected samples by performing a qPCR.
The main applications qualitative analysis by qPCR include:
• Virus and pathogen detection
• GMO detection
• Cultivar identification
• Mutant genotyping
• SNP analysis
Amplification curve and melting curve of target gene
qPCR identification of humanized IL6 transgenic mice
Humanized IL6 transgenic mice are identified by qPCR. If the IL6 gene in transgenic mice are replaced with human IL6 gene, the experiment succeed.
Sample names: IL6 transgenic mouse、Wild-Type mouse
Detected genes: human IL6、mouse IL6、mouse gapdh（reference gene）
Detection method: SYBR Green qPCR method
Test experiment report:
1. Amplification results of human IL6、mouse IL6、mouse gapdh in transgenic mice and wild-type mice.
Amplification curves of human IL6、mouse IL6、mouse gapdh
Melting curves of human IL6、mouse IL6、mouse gapdh
2. Electrophoresis results of human IL6、mouse IL6、mouse gapdh in transgenic mice and wild-type mice.
Electrophoresis results in 2% agarose
3. Mouse IL6 genes have been replaced with human IL6 genes in humanized IL6 transgenic mice.