精品兔单抗

由于免疫系统的独特性,兔单克隆抗体与传统属源单克隆抗体相比具有更好的抗原识别功能。义翘科技采用先进的二代兔单克隆技术倾力打造高质量的抗体,以满足客户的抗体需求。

80%真核重组蛋白免疫
更高的灵敏度和特异性
提供定制服务

兔单抗技术平台

目前商业化的兔单抗技术主要分为两代:第一代以杂交瘤技术为基础,第二代以噬菌体展示技术为基础。义翘科技第二代兔单抗技术经过不断的探索优化形成了自己的独特优势:
1.抗体基因引物优化,确保扩增的高成功率和抗体多样性的获得;
2.建库类型灵活,包括scFv和Fab,库容量≥108
3.固相淘洗、液相淘洗等多种亲和筛选方式,保证阳性克隆率,筛选出高亲和力抗体
4.独有高效瞬时系统保证兔抗体的高水平表达

抗体和抗原之间的平衡解离常数(KD)是指示抗体亲和力的常用指标,KD值越低代表抗体的亲和力越高。已有的大量分析表明传统兔单抗的KD在10-9M左右,义翘科技兔单抗的KD在可以达到10-11M左右,亲和力高于鼠抗10-100倍。

鼠单抗KD=4.950nM 兔单抗KD=0.017nM

义翘科技兔单抗产品具有更高的特异性,检测单一表位,不易于其他蛋白质发生交叉,有助于降低背景干扰。非常适合于条件严苛的IHC应用。与鼠单抗配合使用可以获得同一组织样品上高质量的双染色结果。

胎盘组织,义翘科技CD71兔单克隆抗体 胎盘组织,CD71兔多克隆抗体(供货商A) 胎盘组织,CD71鼠单克隆抗体(供货商B)

义翘科技可提供针对EISA、FCM、IHC-P、WB、ICIF、IP等多种不同应用的兔单克隆抗体产品。

义翘科技兔单克隆抗体一抗结果验证
A.对人胃部组织进行IHC
B.对细胞进行ICC检测
C.对细胞进行流式细胞术
D.对细胞进行WB

小鼠靶标抗原在兔体内的免疫原性要高于小鼠或大鼠。此外,在试用兔单抗小鼠组织进行检测时,兔单抗不存在和小鼠天然抗体的交叉反应,可以避免由此导致的非特异性信号问题。

人分子靶点的部分兔单克隆抗体列表

货号 种属 分子 应用
10084-R015 Human CD274 ELISA, IHC-P
10004-R511 Human ERBB2 ELISA, ELISA(Cap), FCM, lCC/IF, IF
10565-R102 Human CD226 ELISA
10231-R301-P Human LDLR FCM
10259-R110 Human FCGR2B WB, IHC-P, IP
10773-R023 Human ASGR1 ELISA
10004-R002 Human ERBB2 ELISA, IHC-P, FCM, ICC/IF, IF
10001-R021 Human EGFR WB, ELISA, ELISA(Det), ICC/IF, IF, IP
10694-R028 Human EpCAM WB, ELISA, ELISA(Det), IHC-P, FCM, ICC/IF, IF, IP
13909-R226 Human F8 ELISA
10005-R017 Human PVRL2 ELISA
10377-RD67 Human PDCD1 ELISA, IHC-P
10594-RM03 Human PCSK9 ELISA
10186-R125 Human BSG WB, ELISA, IHC-P, FCM, ICC/IF, IF, IP
10136-R111-P Human FLT1 FCM
10390-R024 Human HAVCR2 ELISA, ELISA(Cap)
10390-R108 Human HAVCR2 ELISA
11066-R105 Human VEGFA ELISA
11215-R107 Human LAMP1 WB, ELISA, IHC-P, FCM, ICC/IF, IF, IP
11235-R019 Human REG3A ELISA, IHC-P
10973-R111 Human vWF ELISA, ELISA(Det), IHC-P
11050-R412 Human NGF ELISA
11279-R021 Human CTNNB1 ELISA, IHC-P, ICC/IF, IF, IP
13908-R002 Human ASGR2 ELISA, IHC-P, FCM, ICC/IF, IF
10008-R064 Human VEGFA ELlSA, ELISA(Det)
100087-R013 Human FGF2 HC-P
10014-R101 Human FGF2 ELISA
100393-R024-F Human GPC3 FCM
100393-R024-P Human GPC3 FCM
10084-R001 Human CD274 ELISA
10231-R301-F Human LDLR FCM
10289-R102 Human LGALS3 HC-P
10400-R113 Human CD4 EIISA, IHC-P
10417-R006 Human TNFRSF1B ELISA
10980-R081 Human CD8A HC-P
11051-Rn208 Human HAVCR1 ELISA
12078-R001 Human GLA W8, ELISA, IP
10007-R104 Human CSF3 ELISA
10073-R001 Human CD14 IHC-P
10160-R005 Human CFD ELISA
10259-R112 Human FCGR2B ELISA.ELISA(Det)
10327-RM04 Human MMP9 ELISA
10995-R018 Human SerpinG1 WB, ELISA, ELISA(Det), IP
11031-R039 Human CD8B HC-P
11192-R179 Human CD68 HC-P
11996-R351 Human KIT ELISA, IHC-P
12163-R005 Human VSIG4 ELISA, ELISA(Det)
13908-R002-P Human ASGR2 FCM
100128-R001 Human CD79A IHC-P
100129-R101 Human KRT14 WB, IHC-P, IP
100254-R211 Human VIM IHC-P

小鼠分子靶点的部分兔单克隆抗体列表

货号 种属 分子 应用
50134-R001 Mouse CD4 ELISA, IHC-P
50086-R001 Mouse FCGR1 ELISA, IHC-P
50010-R401 Mouse CD274 FCM
50683-R036 Mouse AG02 IHC-P, IP
50010-R485 Mouse CD274 FCM, ICC/IF, IF
50191-R032 Mouse CD38 EUSA, IHC-P
50413-R001-P Mouse F3 FCM
50083-R103 Mouse ASGR1 ELISA
50129-R004 Mouse MSR1 IHC-P, FCM, ICC/IF, IF
50389-R208 Mouse CD8A FCM.ICC/IFJF
50067-R014 Mouse TNFSF9 ELISA, FCM, ICC/IF, IF
50083-R114 Mouse ASGR1 IHC-P
50469-R110 Mouse IFNAR1 ICC/IF, IF
50036-R011 Mouse FCGR4 ELISA, IHC-P
50073-R167 Mouse IL18 ELISA, ELISA(Det)
50128-R112-A Mouse TNFRSF1B FCM
50305-R032-P Mouse LDLR FCM
50413-R001 Mouse F3 FCM
50440-R020 Mouse ICAM1 ELISA, ELISA[Cap), IHC-P
50030-R005 Mouse Fcgr2 ELISA
50059-R220 Mouse CSF1R ELISA
50086-R008 Mouse FCGR1 EUSA, IHC-P
50086-R027 Mouse FCGR1 FCM, ICC/IF, IF
50110-R034 Mouse CD27 ELISA
50259-R305 Mouse PVR FCM, ICC/IF, IF
50279-R001 Mouse REN ELISA, IHC-P
50331-R038 Mouse ECM1 ELISA
50396-R034 Mouse GRN ELISA, ELISA(Cap)
50589-R013 Mouse CD34 WB, ELISA, ELISA(Det), IHC-P, FCM, ICC/IF, IF, IP
51179-R021 Mouse IL36G ELISA
50010-R485-P Mouse CD274 FCM
50061-R015 Mouse IL13Ra2 ELISA
50073-R169 Mouse IL18 ELISA(Cap)
50086-R027-F Mouse FCGR1 FCM
50114-R024 Mouse IL1A ELISA
50155-R277 Mouse CXCL9 ELISA
50256-R066 Mouse PDPN IHC-P
50362-R003 Mouse F9 ELISA
50389-R208-A Mouse CD8A FCM
50389-R208-F Mouse CD8A FCM
50412-R131 Mouse TNFRSF10B ELISA
50468-R076 Mouse CD55 FCM, ICC/IF, IF
50503-R037 Mouse CTLA4 ELISA
50537-R029 Mouse CD2 ELISA, IHC-P
50660-R001 Mouse CA9 IHC-P
50813-R048 Mouse SLC3A2 IHC-P
51091-R112 Mouse EGFR ELISA
50010-R485-A Mouse CD274 FCM
50023-R503 Mouse CD14 FCM, ICC/IF, IF
50087-R011-P Mouse IL2RG FCM
50110-R133 Mouse CD27 ELISA

其他物种靶点的部分兔单克隆抗体列表

货号 种属 分子 应用
11055-RM05 Influenza HA WB, EUSA, IHC-P, FCM, ICC/IF, IF, IP
11082-R002 Influenza HA ELISA, ELlSA(Cop), FCM, B/N, Microneutralizai-ton(MN), Hemagglutininlnhibition(HI)
86001-RM01 Influenza HA WB, ELISA, IHC-P, FCM, ICC/IF, IF, IP
11055-RM10 Influenza HA WB, IHC-P, FCM, ICC/IF, IF, IP
11058-R001 Influenza HA WB, EUSA, IHC-P, FCM, ICC/IF, IF, IP
40069-R723 MERS-CoV Spike Microneutralizaiton(MN)
80016-R005-F Rat FCGR1 FCM
80016-R015 Rat FCGR1 ELISA
80076-R007 Rat IL6 ELISA
80079-R187 Rat ERBB2 ELISA
11049-R009 RSV RSV-F ELISA, IHC-P, FCM, ICC/IF, IF, IP
13105-R208 Aequorea victoria GFP WB, ELISA, IP
11680-R112 C.perfringens NA ELISA, IHC-P, FCM, ICC/IF, IF, IP
70109-R039 Canine PDCD1 ELISA
70001-R202 Canine HAVCR1 ELISA, IHC-P
10202-R038 CMV gB WB, EUSA, IHC-P, FCM, ICC/IF, IF, IP
90001-R002 Cynomolgus TP53 IHC-P, ICC/IF, IF
90014-R046 Cynomolgus FCGR2B ELISA
40094-R106 EBOV EBOV-G WB, EUSA, IHC-P, FCM, ICC/IF, IF, IP
40094-R301 EBOV EBOV-G ELfSA, IHC-P, FCM, lCC/IF, IF, IP
60004-R071 Ferret MS4A1 ELISA
60001-R073-A Ferret CD8A FCM
60002-R030 Ferret TNF ELISA
40359-R137 H10N8 HA ELISA, IHC-P, FCM, ICC/IF, IF, IP
11684-R016 H1N1 HA ELISA(Cap), IHC-P, FCM
11056-R006 H3N2 HA ELISA, IHC-P, FCM, ICC/IF, IF, IP
11056-R104 H3N2 HA ELISA, IHC-P, FCM, ICC/IF, IF, IP
11056-R014 H3N2 HA ELISA(Cap)
11048-RM01 H5N1 HA WB, EUSA, IHC-P, FCM, ICC/IF, IF, IP
40021-R028 HCoV Spike ELISA, IHC-P, FCM, ICC/IF, IF, IP
11233-R011 HIV gp120 WB, ELISA, ELlSA(Det), IHC-P, FCM, lCC/IF, IF, IP
11695-R002 HIV Gag-p24 WB, ELISA, IHC-P, FCM, ICC/IF, IF, IP
11233-R001 HIV gp120 ELISA, ELlSA(Cap), IHC-P, FCM, lCC/IF, IF, IP