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Rat IgG antibodies have four subclasses: IgG1, IgG2a, IgG2b and IgG2c. Each subclass antibody's binding affinity to staphylococcal protein A with the order of: IgG2c > IgG1 > IgG2b > IgG2a (the affinity of IgG2b being very weak, and IgG2a having virtually no binding affinity for protein A). Rat IgG antibodies are often developed against mouse antigen due to the fact that mouse cannot generate strong immune response to its own proteins.
Sino Biological offers full services for rat IgG antibody production and purification. Two different strategies are provided: rat hybridoma cell culture or transient transfection in mammalian cells. Transient transfection is offered as an alternative approach if the rat hybridoma cell line is not stable, difficult to grow under serum-free condition, or it is contaminated. We are experienced in cloning the rat antibody light and heavy chain genes from the hybridoma cells, then use our advanced transient transfection platform to produce the rat antibodies under serum-free conditions at any scale required by customer. Our rat IgG antibody production and purification service is easy to manage, highly reliable, and affordable. It frees up your time and let you focus your expertise on key elements of your research.
How to Submit a Request for Antibody Production and Purification Service?
If you are interested in using our antibody purification services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CROemail@example.com. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.