SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified)

COVID-19 Spike S1 Research.
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SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified): 产品信息

纯度
> 90 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物活性
1. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) (40591-V08H), the EC50 of SARS-CoV-2/2019-nCoV Spike Protein (S1 Subunit, His tag) (Cat: 40591-V08H) is 200-600 ng/mL.
2. Loaded ACE2 Protein, Human, Recombinant (mFc Tag) (Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat. No. 40591-V08H) with an affinity constant of 47.7 nM as determined by Octet RED System.
蛋白构建
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) spike protein S1 Subunit (YP_009724390.1) (Val16-Arg685) was expressed with a polyhistidine tag at the C-terminus.
表达宿主
HEK293 Cells
种属
2019-nCoV
预测 N 端
Val16
分子量
The recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag) consists of 681 amino acids and predicts a molecular mass of 76.5 kDa.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified): 图片

Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 10μg/mL (100μL/well) can bind 2019-nCoV Spike Protein (S1, His tag) (40591-V08H), the EC50 of 2019-nCoV Spike Protein (S1, His tag) (40591-V08H) is 200-400 ng/mL.
Loaded ACE2 Protein, Human, Recombinant (mFc Tag) (Cat. No. 10108-H05H) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (Cat. No. 40591-V08H) with an affinity constant of 47.7 nM as determined by Octet RED System.

SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein (HPLC-verified): 别称

coronavirus spike Protein, 2019-nCoV; cov spike Protein, 2019-nCoV; ncov RBD Protein, 2019-nCoV; ncov s1 Protein, 2019-nCoV; ncov s2 Protein, 2019-nCoV; ncov spike Protein, 2019-nCoV; NCP-CoV RBD Protein, 2019-nCoV; NCP-CoV s1 Protein, 2019-nCoV; NCP-CoV s2 Protein, 2019-nCoV; NCP-CoV Spike Protein, 2019-nCoV; novel coronavirus RBD Protein, 2019-nCoV; novel coronavirus s1 Protein, 2019-nCoV; novel coronavirus s2 Protein, 2019-nCoV; novel coronavirus spike Protein, 2019-nCoV; RBD Protein, 2019-nCoV; S1 Protein, 2019-nCoV; S2 Protein, 2019-nCoV; Spike RBD Protein, 2019-nCoV

Coronavirus spike 背景信息

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
参考文献
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

2019-nCoV Coronavirus spike 蛋白

  • The Pathogenicity of 2019 Novel Coronavirus in hACE2 Transgenic Mice
    Author
    Bao, L;Deng, W;Huang, B;Gao, H;Ren, L;Wei, Q;Yu, P;
    Year
    2020
    Journal
    bioRxiv
    Application
    ELISA;vaccination
  • Age-related rhesus macaque models of COVID-19
    Author
    Yu, P;Qi, F;Xu, Y;Li, F;Liu, P;Liu, J;Bao, L;Deng, W;Gao, H;Xiang, Z;Xiao, C;Lv, Q;Gong, S;Liu, J;Song, Z;Qu, Y;Xue, J;Wei, Q;Liu, M;Wang, G;Wang, S;Yu, H;Liu, X;Huang, B;Wang, W;Zhao, L;Wang, H;Ye, F;Zhou, W;Zhen, W;Han, J;Wu, G;Jin, Q;Wang, J;Tan, W;Qin, C;
    Year
    2020
    Journal
    Anim Models Exp Med
  • Analysis of SARS-CoV-2 Antibodies in COVID-19 Convalescent Blood using a Coronavirus Antigen Microarray
    Author
    De Assis, RR;Jain, A;Nakajima, R;Jasinskas, A;
    Year
    2020
    Journal
    BioRxiv
  • Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray
    Author
    Khan, S;Nakajima, R;Jain, A;De Assis, RR;Jasinskas, A;
    Year
    2020
    Journal
    BioRxiv
  • Cholesterol Metabolism--Impact for SARS-CoV-2 Infection Prognosis, Entry, and Antiviral Therapies
    Author
    Wei, C;Wan, L;Zhang, Y;Fan, C;Yan, Q;Yang, X;Gong, J;
    Year
    2020
    Journal
    medRxiv
  • Human monoclonal antibodies block the binding of SARS-CoV-2 spike protein to angiotensin converting enzyme 2 receptor
    Author
    Ye, L;Chen, X;Li, R;Pan, Z;Qian, C;Yang, Y;You, R;
    Year
    2020
    Journal
    medRxiv
  • Identification of Human Single-Domain Antibodies against SARS-CoV-2
    Author
    Wu, Y;Li, C;Xia, S;Tian, X;Kong, Y;Wang, Z;Gu, C;Zhang, R;Tu, C;Xie, Y;Yang, Z;Lu, L;Jiang, S;Ying, T;
    Year
    2020
    Journal
    Cell Host Microbe
  • Immunogenicity of a DNA vaccine candidate for COVID-19
    Author
    Smith, TRF;Patel, A;Ramos, S;Elwood, D;Zhu, X;Yan, J;Gary, EN;Walker, SN;Schultheis, K;Purwar, M;Xu, Z;Walters, J;Bhojnagarwala, P;Yang, M;Chokkalingam, N;Pezzoli, P;Parzych, E;Reuschel, EL;Doan, A;Tursi, N;Vasquez, M;Choi, J;Tello-Ruiz, E;Maricic, I;Bah, MA;Wu, Y;Amante, D;Park, DH;Dia, Y;Ali, AR;Zaidi, FI;Generotti, A;Kim, KY;Herring, TA;Reeder, S;Andrade, VM;Buttigieg, K;Zhao, G;Wu, JM;Li, D;Bao, L;Liu, J;Deng, W;Qin, C;Brown, AS;Khoshnejad, M;Wang, N;Chu, J;Wrapp, D;McLellan, JS;Muthumani, K;Wang, B;Carroll, MW;Kim, JJ;Boyer, J;Kulp, DW;Humeau, LMPF;Weiner, DB;Broderick, KE;
    Year
    2020
    Journal
    Nat Commun
  • Kinetics of SARS-CoV-2 specific IgM and IgG responses in COVID-19 patients
    Author
    Sun, B;Feng, Y;Mo, X;Zheng, P;Wang, Q;Li, P;Peng, P;Liu, X;Chen, Z;Huang, H;Zhang, F;Luo, W;Niu, X;Hu, P;Wang, L;Peng, H;Huang, Z;Feng, L;Li, F;Zhang, F;Li, F;Zhong, N;Chen, L;
    Year
    2020
    Journal
    Emerg Microbes Infect
  • Lectin-like Intestinal Defensin Inhibits 2019-nCoV Spike binding to ACE2
    Author
    Wang, C;Wang, S;Li, D;Zhao, X;Han, S;Wang, T;Zhao, G;
    Year
    2020
    Journal
    bioRxiv
  • Ocular conjunctival inoculation of SARS-CoV-2 can cause mild COVID-19 in Rhesus macaques
    Author
    Deng, W;Bao, L;Gao, H;Xiang, Z;Qu, Y;Song, Z;Gong, S;
    Year
    2020
    Journal
    bioRxiv
    Application
    Elisa
  • Rapid and quantitative detection of COVID-19 markers in micro-liter sized samples
    Author
    Tan, X;Lin, C;Zhang, J;Oo, MKK;Fan, X;
    Year
    2020
    Journal
    bioRxiv
    Application
    microfluidic ELISA platform
  • Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based Biosensor
    Author
    Seo, G;Lee, G;Kim, MJ;Baek, SH;Choi, M;Ku, KB;Lee, CS;Jun, S;Park, D;Kim, HG;Kim, SJ;Lee, JO;Kim, BT;Park, EC;Kim, SI;
    Year
    2020
    Journal
    ACS Nano
  • Rhesus macaques can be effectively infected with SARS-CoV-2 via ocular conjunctival route
    Author
    Deng, W;Bao, L;Gao, H;Xiang, Z;Qu, Y;Song, Z;Gong, S;
    Year
    2020
    Journal
    bioRxiv
    Application
    ELISA
  • SARS-CoV-2 and SARS-CoV Spike-RBD Structure and Receptor Binding Comparison and Potential Implications on Neutralizing Antibody and Vaccine Development
    Author
    Xie, L;Sun, C;Luo, C;Zhang, Y;Zhang, J;Yang, J;Chen, L;
    Year
    2020
    Journal
    bioRxiv
  • Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins using High-Resolution Mass Spectrometry
    Author
    Zhang, Y;Zhao, W;Mao, Y;Wang, S;Zhong, Y;Su, T;
    Year
    2020
    Journal
    bioRxiv
  • Ultra-Sensitive High-Resolution Profiling of Anti-SARS-CoV-2 Antibodies for Detecting Early Seroconversion in COVID-19 Patients
    Author
    Norman, M;Gilboa, T;Ogata, AF;Maley, AM;Cohen, L;
    Year
    2020
    Journal
    medRxiv
    Application
    Bead Conjugation
  • Reinfection could not occur in SARS-CoV-2 infected rhesus macaques
    Author
    Bao, L;Deng, W;Gao, H;Xiao, C;Liu, J;Xue, J;Lv, Q;Liu, J;
    Year
    2020
    Journal
    bioRxiv
    Application
    ELISA
  • SARS-CoV-2-specific antibody detection for sero-epidemiology: a multiplex analysis approach accounting for accurate seroprevalence
    Author
    den Hartog, G;Schepp, R;Kuijer, M;GeurtsvanKessel, C;van Beek, J;Rots, N;Koopmans, M;van der Klis, F;van Binnendijk, R;
    Year
    2020
    Journal
    medRxiv
    Application
    bead-based multiplex immunoassays
  • High-Accuracy Multiplexed SARS-CoV-2 Antibody Assay with Avidity and Saliva Capability on a Nano-Plasmonic Platform
    Author
    Liu, T;Hsiung, J;Zhao, S;Kost, J;Sreedhar, D;Olson, K;Keare, D;Roche, J;Hanson, C;Press, C;Boggs, J;Rodriguez-Soto, J;Montoya, J;Tang, M;Dai, H;
    Year
    2020
    Journal
    bioRxiv
    Application
    microarray
  • A rapidly adaptable biomaterial vaccine for SARS-CoV-2
    Author
    Langellotto, F;Seiler, B;Yu, J;Cartwright, M;White, D;Yeager, C;Super, M;Doherty, E;Barouch, D;Mooney, D;
    Year
    2020
    Journal
    bioRxiv
  • A Targeted Vaccine against COVID-19: S1-Fc Vaccine Targeting the Antigen-Presenting Cell Compartment Elicits Protection against SARS-CoV-2 Infection
    Author
    Herrmann, A;Maruyama, J;Yue, C;Lahtz, C;Zhou, H;Kerwin, L;Guo, W;Zhang, Y;Hoo, W;Yei, S;Kwon, S;Fu, Y;Johnson, S;Ledesma, A;Zhou, Y;Zhuang, Y;Yei, E;Adamus, T;Praessler, S;Ji, H;
    Year
    2020
    Journal
    bioRxiv
    Application
    Elisa
  • Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins
    Author
    Zhang, Y;Zhao, W;Mao, Y;Chen, Y;Wang, S;Zhong, Y;Su, T;Gong, M;Du, D;Lu, X;Cheng, J;Yang, H;
    Year
    2020
    Journal
    bioRxiv
  • Cell-Mimicking Nanodecoys Protect Lung Cells by Neutralization of SARS-CoV-2 Spike Proteins
    Author
    Li, Z;Dinh, P;Popowski, K;Lutz, H;Wang, Z;Zhu, D;Hu, S;Lobo, J;Cheng, K;
    Year
    2020
    Journal
    Research Square
    Application
    SARS-CoV-2 Mimicking Virus
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