SARS-CoV-2 (2019-nCoV) Plpro/papain-like protease-His recombinant protein (aa 1564-1880)

COVID-19 Plpro Research.
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SARS-CoV-2 (2019-nCoV) Plpro/papain-like protease-His recombinant protein (aa 1564-1880): 产品信息

纯度
> 90 % as determined by SDS-PAGE.
内毒素
Please contact us for more information.
生物活性
Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC)(Bachem, Catalog #I-1690).The Specific Activity is >80pmols/min/μg
蛋白构建
A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) papain-like protease (YP_009725299.1) (Glu1564-Val1880) was expressed with a polyhistidine tag at the N-terminus.
表达宿主
E.coli
种属
2019-nCoV
预测 N 端
Met
分子量
The recombinant SARS-CoV-2 (2019-nCoV) Plpro / papain-like protease (His Tag) consists of 324 amino acids and predicts a molecular mass of 36.79 kDa.
缓冲液
Lyophilized from sterile 20mM Tris 500mM NaCl, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Plpro/papain-like protease 背景信息

The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.
参考文献
    Yahira M. Báez-Santos.The SARS-coronavirus papain-like protease: Structure, function and inhibition by designed antiviral compounds. Antiviral Research.2015
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