Angiopoietin-2 Protein, Human, Recombinant (His Tag)

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Angiopoietin-2 Protein, Human, Recombinant (His Tag): 产品信息

纯度
> 92 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured by its ability to bind recombinant human Tie2-His in a functional ELISA .
2. Measured by its ability to bind recombinant human Tie2-Fch in a functional ELISA.
蛋白构建
A DNA sequence encoding the human Angiopoietin 2 (NP_001138.1) (Met 1-Phe 496) was expressed, fused with a polyhistidine tag at the C-terminus.
NP No.
表达宿主
HEK293 Cells
种属
Human
预测 N 端
Tyr 19
分子量
The recombinant human Ang2 consists of 489 amino acids and predicts a molecular mass of 56.4 kDa. As a result of glycosylation, the rhAng2 migrates as approximately 63.5 kDa band in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile 2mM MOPS, 15mM NaCl, .5% CHAPS, pH 7.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Angiopoietin-2 Protein, Human, Recombinant (His Tag): 图片

Citation List

Angiopoietin-2 Protein, Human, Recombinant (His Tag): 别称

AGPT2 Protein, Human; ANG2 Protein, Human; Angiopoietin-2 Protein, Human

Angiopoietin-2 背景信息

Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.
全称
angiopoietin 2
研究领域
参考文献
  • Thomas M, et al. (2009) The role of the Angiopoietins in vascular morphogenesis. Angiogenesis. 12(2): 125-37.
  • Hu B, et al. (2009) Angiopoietin-2: development of inhibitors for cancer therapy. Curr Oncol Rep. 11(2): 111-6.
  • Fiedler U, et al. (2006) Angiopoietins: a link between angiogenesis and inflammation. Trends Immunol. 27: 552-8.
  • Escobar E, et al. (2004) Angiotensin II, cell proliferation and angiogenesis regulator: biologic and therapeutic implications in cancer. Curr Vasc Pharmacol. 2(4): 385-99.
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